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M. D. Pinazo-Duran, R. Gallego-Pinazo, V. Zanon-Moreno, S. Pons-Vazquez, J. J. Garcia-Medina, M. Serrano; Mechanisms Underlying p53 Genetic Regulation of Vascular Endothelial Growth Factor (VEGF) and Brain Derived Neurotrophic Factor (BDNF) in Mice Retina. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4101.
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The extent of angiogenesis and/or VEGF expression in vitreo-retinal diseases correlates with poor visual prognosis. Among the genes up-regulated by VEGF was the brain-derived growth factor (BDNF). The aim of this study was to analyse retinal changes in VEGF/BDNF expression, in relation to p53 gene availability.
Homogenates of mice retina-choroid were obtained from one-year-old mice distributed into two groups: 1) mice carrying two extra copies of p53 (in addition to the two endogenous alleles) in the form of large genomic transgenes (sp53G; n=6), and 2) wild type mice with normal p53 genetic background (CG); n=8). The VEGF and BDNF expression was assayed by enzyme-linked immunosorbent assays (ELISAs). Data processing was done by the SPSS 15.0 program.
Data demonstrate distinct expression of VEGF (85,14 ± 5,73 vs 73,52 ± 4,16 p=0,0168) and especially of BDNF (121,13 ± 2,72 vs 108,16 ± 4,43; p=0,0025) in the sp53 mice retinas than in the controls.
Considering the multipotent actions of VEGF, and the fact that inhibiting BDNF expression may down-regulates VEGF secretion and angiogenesis, our findings strongly suggest that p53 may have some role in the progression of human choroidal neovascularization.
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