April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Serum Matrix Metalloproteinase Profile in Neovascular Age Related Macular Degeneration
Author Affiliations & Notes
  • T. Adewoyin
    Ophthalmology, Institute Of Ophthalmology, London, United Kingdom
  • E. Fletcher
    Ophthalmology, Buckinghamshire NHS Trust, Marlow, United Kingdom
  • S. Sivaprasad
    Ophthalmology, King's College Hospital, London, United Kingdom
  • V. L. Calder
    Ocular Biology & Therapeutics, UCL Institute of Ophthalmology, London, United Kingdom
  • K. Gregory-Evans
    Ophthal & Visual Sciences, University of British Columbia, British Columbia, British Columbia, Canada
  • V. Chong
    Ophthalmology, Oxford Eye Hospital, Oxford, United Kingdom
  • Footnotes
    Commercial Relationships  T. Adewoyin, None; E. Fletcher, None; S. Sivaprasad, None; V.L. Calder, None; K. Gregory-Evans, None; V. Chong, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 4110. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      T. Adewoyin, E. Fletcher, S. Sivaprasad, V. L. Calder, K. Gregory-Evans, V. Chong; Serum Matrix Metalloproteinase Profile in Neovascular Age Related Macular Degeneration. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4110.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: : Age related macular degeneration (AMD) is a complex disorder with a multifactorial aetiology. Structural changes in Bruch’s membrane occur in AMD. Extracellular matrix dysregulation, due to aberrant activity of matrix metalloproteinases (MMPs) and their inhibitors, might play a role in the pathogenesis of AMD. HTRA1 is associated with AMD. The exact mechanism remains unknown. As it was suggested that HTRA1 might be a serine proteinases, we would like to assess whether HTRA1 "at risk" alleles would alter MMPs levels.

Methods: : Two hundred and forty eight Caucasian patients were recruited. Classification of the cohort into controls, drusen and neovascular AMD was made by biomicroscopy and fundal photography. Genotyping of the single nucleotide polymorphism for HtrA1 rs11200638 was also performed. Serum samples were analysed to determine the MMP profile (7 MMP’s, 3 TIMPS’s) using a human MMP protein microarray .

Results: : MMP 9 and MMP10 was significantly raised in patients positive for the risk allele (p<0.02, p<0.09 respectively)There was also a significant difference in MMP 1, 8, TIMP2 & 4 levels in drusen (p=0.016, p=0.029, p=0.002, p=0.034 and AMD groups (p=0.004,p= 0.010, p<0.00001,p=0.002 ,p=0.04) compared to control.

Conclusions: : AMD pathogenesis is complex and not fully elucidated at present. HtrA1 has been shown to confer an increased risk of AMD, and influences the expression of metalloproteinases. Identification of biomarkers that vary in disease states can help with prediction and prognosis as well as developing novel therapy.

Keywords: age-related macular degeneration • retina • Bruch's membrane 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.