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P. Fernandez, S. Recalde, M. Moreno-Orduña, A. Salinas, J. Zarranz-Ventura, J. M. Caire, A. Garcia-Layana; Analysis of Circulating Markers for Age-Related Macular Degeneration. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4111.
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One of the main inconveniences for early diagnosis of AMD is the lack of systemic biomarkers. Plasminogen Activator Inhibitor (PAI-1) is considered an anti-angiogenic factor and C-Reactive Protein (CRP) is an inflammation marker. We aimed to measure plasmatic levels on AMD patients for several molecules and compare with control individuals.
Eighty patients with wet AMD (with choroidal neovascularization at least in one eye) and 79 age and sex-matched healthy controls were selected for this study. Plasma-EDTA was isolated from peripheral blood and used for all molecules determinations. Enzyme-linked immunosorbent assays (ELISA) were assessed to quantify systemic levels for high sensitivity CRP and PAI-1. All analyses were performed following manufacturers’ instructions. Samples were tested for normality and after that subjected to t-student analysis for independent samples (SPSS v.15.0).
CRP levels were significantly increased in plasma of AMD patients compared with controls (P<0.001). In contrast, levels of PAI-1 were significantly lower in AMD individuals compared with controls (P=0.0013).
These results suggest that circulating levels of inflammatory and pro-angiogenic molecules such as PAI-1 and CRP are related to AMD. CRP is elevated in AMD indicating the inflammatory role of the disease. Angiogenic status of AMD is confirmed by a decrease in PAI-1 levels in AMD patients. It is necessary to further analyse whether that molecules could be accurate biomarkers for AMD, and investigate other factors such as matrix metaloproteinases to use as a screening tool.
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