April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Analysis of Circulating Markers for Age-Related Macular Degeneration
Author Affiliations & Notes
  • P. Fernandez
    Experimental Ophthalmology Laboratory, University of Navarra, Pamplona Navarra, Spain
  • S. Recalde
    Experimental Ophthalmology Laboratory, University of Navarra, Pamplona Navarra, Spain
  • M. Moreno-Orduña
    Experimental Ophthalmology Laboratory, University of Navarra, Pamplona Navarra, Spain
  • A. Salinas
    Ophthalmology, Clinica Universidad de Navarra, Pamplona Navarra, Spain
  • J. Zarranz-Ventura
    Ophthalmology, Clinica Universidad de Navarra, Pamplona Navarra, Spain
  • J. M. Caire
    Ophthalmology, Clinica Universidad de Navarra, Pamplona Navarra, Spain
  • A. Garcia-Layana
    Experimental Ophthalmology Laboratory, University of Navarra, Pamplona Navarra, Spain
    Ophthalmology, Clinica Universidad de Navarra, Pamplona Navarra, Spain
  • Footnotes
    Commercial Relationships  P. Fernandez, None; S. Recalde, None; M. Moreno-Orduña, None; A. Salinas, None; J. Zarranz-Ventura, None; J.M. Caire, None; A. Garcia-Layana, None.
  • Footnotes
    Support  Supported in part by a grant of PIUNA (Proyectos de Investigación Universidad de Navarra, Fundación Universidad de Navarra) and Caja Navarra "Tú eliges, tú decides" (#16513) projects.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 4111. doi:
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      P. Fernandez, S. Recalde, M. Moreno-Orduña, A. Salinas, J. Zarranz-Ventura, J. M. Caire, A. Garcia-Layana; Analysis of Circulating Markers for Age-Related Macular Degeneration. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4111.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : One of the main inconveniences for early diagnosis of AMD is the lack of systemic biomarkers. Plasminogen Activator Inhibitor (PAI-1) is considered an anti-angiogenic factor and C-Reactive Protein (CRP) is an inflammation marker. We aimed to measure plasmatic levels on AMD patients for several molecules and compare with control individuals.

Methods: : Eighty patients with wet AMD (with choroidal neovascularization at least in one eye) and 79 age and sex-matched healthy controls were selected for this study. Plasma-EDTA was isolated from peripheral blood and used for all molecules determinations. Enzyme-linked immunosorbent assays (ELISA) were assessed to quantify systemic levels for high sensitivity CRP and PAI-1. All analyses were performed following manufacturers’ instructions. Samples were tested for normality and after that subjected to t-student analysis for independent samples (SPSS v.15.0).

Results: : CRP levels were significantly increased in plasma of AMD patients compared with controls (P<0.001). In contrast, levels of PAI-1 were significantly lower in AMD individuals compared with controls (P=0.0013).

Conclusions: : These results suggest that circulating levels of inflammatory and pro-angiogenic molecules such as PAI-1 and CRP are related to AMD. CRP is elevated in AMD indicating the inflammatory role of the disease. Angiogenic status of AMD is confirmed by a decrease in PAI-1 levels in AMD patients. It is necessary to further analyse whether that molecules could be accurate biomarkers for AMD, and investigate other factors such as matrix metaloproteinases to use as a screening tool.

Keywords: age-related macular degeneration • clinical laboratory testing • clinical (human) or epidemiologic studies: risk factor assessment 
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