April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Proliferative and Inflammatory Response in Three Rat Models of Lacrimal Gland Damage and Repair
Author Affiliations & Notes
  • E. B. Machado Filho
    Hospital das Clinicas da Faculdade de Me, Ribeirao Preto, Brazil
  • T. M. Batista
    Departamento de Fisiologia, Insituto de Biologia, Unicamp, Campinas, SP, Brazil
  • C. M. Módulo
    Hospital das Clinicas da Faculdade de Me, Ribeirao Preto, Brazil
  • C. Dias
    Hospital das Clinicas da Faculdade de Me, Ribeirao Preto, Brazil
  • L. T. Malki
    Hospital das Clinicas da Faculdade de Me, Ribeirao Preto, Brazil
  • M. T. B. Machado
    Hospital das Clinicas da Faculdade de Me, Ribeirao Preto, Brazil
  • E. M. Rocha
    Hospital das Clinicas da Faculdade de Me, Ribeirao Preto, Brazil
  • Footnotes
    Commercial Relationships  E.B. Machado Filho, None; T.M. Batista, None; C.M. Módulo, None; C. Dias, None; L.T. Malki, None; M.T.B. Machado, None; E.M. Rocha, None.
  • Footnotes
    Support  CNPq, FAPESP, FAEPA
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 4184. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      E. B. Machado Filho, T. M. Batista, C. M. Módulo, C. Dias, L. T. Malki, M. T. B. Machado, E. M. Rocha; Proliferative and Inflammatory Response in Three Rat Models of Lacrimal Gland Damage and Repair. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4184.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : The aim of this study was to evaluate the lacrimal gland (LG) and ocular surface (OS) response to LG partial ablation (G1), allogenic transplantation (G2) and denervation (G3).

Methods: : Eight-week-old male Wistar rats were submitted to the following procedure in the right LG: G1) partial ablation, G2) partial ablation and transplantation of an allogenic LG, G3) denervation. In all groups the left eye was the control (n=5-10/group). After 1 and 2 months, the animals were anesthetized and evaluated by slit-lamp and phenol red thread test. Tissues were removed and processed for histology and western blot. PCNA and Caspase-3 expression were evaluated for proliferative and apoptotic activity.

Results: : Slit-lamp observation revealed inflammation and corneal vascularization in G2 and mild to moderate OS damage in G1 and G3. The levels of phenol red thread test did not differ among groups after 1 and 2 months (p<0.05). The LG weight was significantly lower in G1 and G2 after 1 and 2 months (p=0,0386 for 1 month and P<0.0001 for 2 month). The expression for Caspase-3 was similar among the groups after 1 month (p=0.346) and PCNA was significantly higher in G1 (p=0,0286).

Conclusions: : The present findings indicate that LG removal and transplantation induce OS inflammation in rats. The higher expression of PCNA in G1 suggests higher proliferative activity in this model. Overall, our work offer substrate for further studies of LG injury and repair related to dry eye syndrome and OS injury.

Keywords: cornea: tears/tear film/dry eye • lacrimal gland • inflammation 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×