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B. Gray, L. C. Huang, C. Garufis, A. Gwon, J. R. Martiz; Biometric Analysis of the Rabbit Cornea - A Potential Model for Refractive Surgical Technology. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4200.
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© ARVO (1962-2015); The Authors (2016-present)
The rabbit is a common model used to evaluate pre-clinical safety and performance of medical devices in ophthalmic research. This study aims to establish baseline corneal biometric measurements in native New Zealand white rabbits.
Twenty New Zealand white rabbits (average weight of 2.4kg) were examined for corneal power, pachymetry and endothelial cell density. The Pentacam HR Scheimpflug camera (Oculus) was used to analyze the keratometry (K1, K2, Mean K and dK values), the central corneal thickness, and overall thinnest location of the cornea. Two additional instruments, the IOPac (Reichert) and the Visante Optical Coherence Tomographer (OCT, Zeiss) were also utilized to determine central corneal thickness. The corneal endothelial cell count was determined using the NIDEK Confoscan 4 specular microscope.
. The keratotomy values of rabbit eyes examined were as follows (n=40): K1= 48.2 ± 2.0 D, K2 = 49.5 ± 1.9 D, Mean K = 48.8 ± 1.9 D, and dK = 1.3 ± 1.0 D. Analysis by Pentacam showed that the average rabbit central corneal thickness was 353 ± 35 µm, and the overall minimum corneal thickness averaged 311 ± 55 µm. Rabbit central corneal thickness when measured by IOPac was 364 ± 19 µm and when evaluated by OCT was 354 ± 27 µm. Rabbit central corneal thickness measurements obtained from the three ophthalmic instruments were not significantly different. The endothelial cell count average= 3035 ± 180 cells/mm2.
This study demonstrates the precision and consistency of rabbit central corneal thickness measurements as determined by Pentacam, OCT, and IOPac. Results from this study support literature-reported values of rabbit central corneal thickness obtained with ultrasound and of corneal power by topography, and establish a foundation of native rabbit corneal characteristics, which may serve as a baseline comparison and prove to be a useful pre-clinical model for future refractive surgical studies.
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