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D. C. Lozano, M. D. Twa; Intersession Repeatability of Retinal SD-OCT Imaging in the Brown Norway Rat. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4398.
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© ARVO (1962-2015); The Authors (2016-present)
In vivo retinal imaging by Spectral Domain Optical Coherence Tomography (SD-OCT) has the potential to reveal some of the earliest structural changes in ocular disease. The purpose of this study was to quantify the repeatability of measureable retinal features observed in two consecutive imaging sessions in the normal brown Norway rat.
Retinal imaging was performed in four male brown Norway rats (average weight = 408 g; age = 12 m; n = 4 eyes) using the Spectralis SD-OCT system; this was followed by a second imaging session 6 days later. A six-axis positioning stage was used to align the eye with the imaging system. Axial line scans were collected in both the superior and inferior retina at several locations proximal to the optic nerve head (1.3 to 3.0 mm). Baseline scanning laser ophthalmoscopy images were used as a basis to align scans performed during the follow-up session. The combined nerve fiber/ retinal ganglion cell layer (RGCL) and retinal epithelial layers were segmented from axial B-scan images using custom Matlab routines. The difference in RGCL layer thickness and total retinal thickness between imaging sessions was calculated (mean ± SD) along with the 95% limits of agreement (LoA).
RGCL and total retinal thickness differences in the superior retina were 1.188 ± 2.236 µm (LoA: -8.094 to 10.470 µm) and 0.963 ± 11.793 µm (LoA: -7.588 to 9.514 µm), and for the inferior retina, 1.559 ± 2.114 µm (LoA: -9.484 to 9.624 µm) and -2.550 ± 2.016 µm (LoA: -12.694 to 7.594 µm).
Our results demonstrate that the between-session repeatability of RGCL and total retinal thickness measurements by in-vivo SD-OCT is excellent with limits of agreement that range between 1-3 pixels.
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