April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Vitreous Volume of the Mouse Measured by Quantitative High-Resolution MRI
Author Affiliations & Notes
  • H. J. Kaplan
    Ophthal & Vis Science, University of Louisville, Louisville, Kentucky
  • C.-W. Chiang
    Radiology-Rad Sciences,
    Washington University, St Louis, Missouri
  • J. Chen
    Internal Medicine - Cardiovascular Divison,
    Washington University, St Louis, Missouri
  • S.-K. Song
    Radiology, Washington University, St. Louis, Missouri
  • Footnotes
    Commercial Relationships  H.J. Kaplan, None; C.-W. Chiang, None; J. Chen, None; S.-K. Song, None.
  • Footnotes
    Support  1) Research to Prevent Blindness, NYC, NY; Kentucky Challenge Research Trust Fund; 2) NIH, R21, EYE 018914
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 4414. doi:
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    • Get Citation

      H. J. Kaplan, C.-W. Chiang, J. Chen, S.-K. Song; Vitreous Volume of the Mouse Measured by Quantitative High-Resolution MRI. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4414.

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      © ARVO (1962-2015); The Authors (2016-present)

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The mouse eye has been used for many important pathophysiologic studies of visually-significant diseases. Experimental paradigms frequently involve the injection of diagnostic and/or therapeutic agents into the vitreous cavity, and the analysis of data is dependent on the volume of the vitreous cavity. We assessed the variation in size of the vitreous cavity in 2-month old mice using high-resolution MRI.


The vitreous volume of five 2-month old male C57BL/6 mice was measured by high resolution MRI at 11.7T. Multi-slice spin-echo image covering the entire left eye were acquired at 100 x 100 x 100 µm3 resolution (TR 1 sec, TE 35 ms, average 4). Vitreous was manually identified on each image as the region exhibiting higher intensity than lens, iris, and retina (Fig 1 A&B). To minimize the partial volume effect on vitreous volume measurement, the voxel volume at the edge of vitreous cavity was weighted by a factor of 0.5.


The average volume of the vitreous cavity in 2-month old mouse eyes has a 15% variation (Fig. 1C, mean = 4.4 ± 0.7 µl). Furthermore, the difference in vitreous cavity size is not correlated to the weight of the mouse (R2 = 0.17).


The vitreous volume of the mouse eye can vary significantly in mice of the same age, and is not related to weight of the mouse. Therefore, accurate conclusions concerning the dose effect of an intravitreal injection in the murine vitreous cavity may be biased by the variation in size of the vitreous cavity.  

Keywords: imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • anatomy 

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