April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Examining a Role for Melanin in Retinal Ion Regulation and Neovascularization in Experimental ROP
Author Affiliations & Notes
  • B. A. Berkowitz
    Anatomy/Cell Biol & Ophthal,
    Wayne State Univ Sch of Med, Detroit, Michigan
  • U. B. Kompella
    Pharmaceutical Sciences & Ophthalmology, University of Colorado Denver, Aurora, Colorado
  • D. Bissig
    Anatomy/Cell Biol,
    Wayne State Univ Sch of Med, Detroit, Michigan
  • C. Durairaj
    Pharmaceutical Sciences & Ophthalmology, University of Colorado Denver, Aurora, Colorado
  • R. Roberts
    Anatomy/Cell Biol,
    Wayne State Univ Sch of Med, Detroit, Michigan
  • Footnotes
    Commercial Relationships  B.A. Berkowitz, None; U.B. Kompella, None; D. Bissig, None; C. Durairaj, None; R. Roberts, None.
  • Footnotes
    Support  EY018109 (BAB); JDRF (BAB); AMDCC/MMPC (BAB); RPB (Kresge Eye Institute); and EY017533 (UBK)
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 4460. doi:
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    • Get Citation

      B. A. Berkowitz, U. B. Kompella, D. Bissig, C. Durairaj, R. Roberts; Examining a Role for Melanin in Retinal Ion Regulation and Neovascularization in Experimental ROP. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4460.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Coordinated retinal ion flux is critical for proper vision, whereas retinal ion dysregulation is linked with neovascularization (NV). We tested the hypothesis that the ion buffer melanin in RPE/choroid, influences intraretinal uptake of manganese, a biomarker of regulation of ions like calcium, in control rats, as well as NV severity in retinopathy of prematurity (ROP) rats.

Methods: : In dark adapted, awake and freely moving control rats (Brown Norway (BN, postnatal day 50 (P50)), Long Evans (LE, P20, P50), and Sprague Dawley (SD, P14, P20, P50)), and experimental rats (LE rats 6 and 36 days after 50/10 oxygen exposure, and SD rats 0, 6, and 36 days after 50/10) central retinal ion regulation was measured using manganese-enhanced MRI (MEMRI, MnCl2 ip). In select groups, RPE/choroid melanin content, NV incidence and severity, and visual performance (optokinetic tracking) were evaluated.

Results: : In P50 controls, manganese uptake in RPE/choroid correlated with melanin content (BN > LE > SD); uptake in ONL and inner retina (BN > SD >> LE) did not. In experimental ROP, NV severity (LE > SD ~ BN) did not correlate with melanin levels. 50/10 LE, but not SD, pups had supernormal inner and outer retinal uptake through P50. Supernormal intraretinal uptake in LE rats was associated with subnormal visual performance.

Conclusions: : As expected, melanin content and manganese uptake were linked in RPE/choroid. Melanin levels were not correlated with ion regulation in the rest of the retina, or with NV. NV severity and visual performance impairment were closely associated with apparent intraretinal depolarization.

Keywords: neovascularization • imaging/image analysis: non-clinical • visual acuity 
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