April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
The BALB/cByJ Tyrosinase Allele is Linked to Resistance to Vaso-Obliteration in the Oxygen-Induced Retinopathy Model
Author Affiliations & Notes
  • B. E. O'Bryhim
    Molecular and Integrative Physiology,
    University of Kansas Medical Center, Kansas City, Kansas
  • H. Niu
    Ophthalmology, Kansas University Medical Center, Prairie Village, Kansas
  • J. D. Radel
    Department of Occupational Therapy Education,
    University of Kansas Medical Center, Kansas City, Kansas
  • R. C. Symons
    Ophthalmology, Kansas University Medical Center, Prairie Village, Kansas
  • Footnotes
    Commercial Relationships  B.E. O'Bryhim, None; H. Niu, None; J.D. Radel, None; R.C. Symons, None.
  • Footnotes
    Support  Institutional start up grant from Kansas University Medical Center to Dr. Symons, Knights Templar Eye Foundation Grant QS847250, KS Lions Sight Foundation 35893
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 4471. doi:
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      B. E. O'Bryhim, H. Niu, J. D. Radel, R. C. Symons; The BALB/cByJ Tyrosinase Allele is Linked to Resistance to Vaso-Obliteration in the Oxygen-Induced Retinopathy Model. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4471.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

Retinopathy of prematurity (ROP) is a common cause of blindnessin children. The development and severity of ROP is known tobe influenced by gestational age at delivery, birth weight,and over-use of oxygen in the nursery. It is unknown whethergenetic factors influence ROP severity. This study aims to usethe mouse genetics to identify genes which play a role in thedevelopment of oxygen induced retinopathy (OIR), a model ofROP.

 
Methods:
 

A mapping cross was established between BALB/cByJ and C57BL/6ByJ,which are, respectively, relatively resistant and susceptibleto central retinal vaso-obliteration in the OIR model. So far,111 neonatal F2 mice, out of a planned 200, have been subjectedto 120 hours of 75% inspired O2, beginning on day 7 of neonatallife. 96 hours after the return to room air, mice were anesthetized,perfused with fluorescein-isothiocyanate (FITC)-labeled dextranand enucleated. Retinas were excised and flat-mounted, and theavascular area was measured using ImageJ software.

 
Results:
 

Susceptibility to the development of ROP was not shown to beassociated with sex(p > 0.05), but was shown to correlatewith weight at P16 (p < 10-15) and with albino versus non-albinocoat color (p <10-5).

 
Conclusions:
 

In this cross, mouse weight and allelotype at the tyrosinaselocus were associated with susceptibility to vaso-obliteration.However, these factors explain only a small proportion of thetotal variation, and it is expected that whole genome analysiswill lead to the discovery of further loci.  

 

 
Keywords: retinopathy of prematurity • gene mapping • neovascularization 
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