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I.-Y. Chung, S. Seo, J. Park, J. Yoo; Preconditioning With Triamcinolone Acetonide Prevents Decreased Retinal Levels of Decorin in a Rat Model of Oxygen-Induced Retinopathy. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4483.
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To investigate the effect of triamcinolone acetonide (TA) on retinal expression of decorin in a rat model of oxygen-induced retinopathy(OIR).
OIR was stimulated by exposing Sprague-Dawley (SD) rats to hyperoxia (80 ± 1.3% O2) from postnatal day (P) 2 to P14 and then returning them to normoxia (room air, 21 ± 1.5% O2). Control rats were maintained in normoxia. At P15, TA (40 mg/ml) was injected into the right vitreous of OIR rats and saline into the left vitreous of control rats. All rats were sacrificed at P18. RT-PCR, western blot and immunohistochemistry were performed to detect the effects of TA on molecular and morphological changes in retinal decorin levels in P18 OIR rats.
Retinal levels of decorin mRNA and protein in P18 OIR rats were significantly less than in control rats. In addition, retinal decorin-immunoreactivity was increased in neuronal cells from the ganglion cell layer (GCL) and inner nuclear layer (INL) and decreased in ganglion cells of OIR rats relative to controls. However, pretreatment with TA prevented the decrease in retinal decorin in P18 OIR rats.
Our results suggest that decorin is involved in hypoxic retinal damage and regeneration, and that TA protects retinal neurons damaged by relative hypoxia from decreased decorin levels.
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