April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Temporal and Spatial Progression of Cataracts in the Ephrin-A5 Mutant Lens
Author Affiliations & Notes
  • A. I. Son
    Neuroscience,
    Rutgers Univ, State University of NJ, Piscataway, New Jersey
  • M. Cooper
    Neuroscience, Yale University, New Haven, Connecticut
  • Y. Sun
    Chemical Biology,
    Rutgers Univ, State University of NJ, Piscataway, New Jersey
  • R. Zhou
    Chemical Biology, Rutgers University, Piscataway, New Jersey
  • Footnotes
    Commercial Relationships  A.I. Son, None; M. Cooper, None; Y. Sun, None; R. Zhou, None.
  • Footnotes
    Support  NIH Grant EY019012 and NIH Grant AG032806
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 4609. doi:
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    • Get Citation

      A. I. Son, M. Cooper, Y. Sun, R. Zhou; Temporal and Spatial Progression of Cataracts in the Ephrin-A5 Mutant Lens. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4609.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The crystalline lens requires careful co-ordination of multiple signaling molecules to ensure proper development and maintain transparency. Disruptions in the control of these factors may result in disturbances leading to cataracts. Recently, we and several other studies have shown that the Eph family of receptor tyrosine kinase EphA2 and its ligand ephrin-A5 play an important role in lens development and function. Mutations in both the receptor and the ligand result in cataract formation. To elucidate the cellular mechanisms underlying the development of cataracts, we have identified the timing and location of the lens deficits in ephrin-A5-null mice.

Methods: : Wild-type and ephrin-A5-null eyes at various ages were fixed, sectioned, and examined using H&E staining. Protein expression and localization were determined using immunohistochemistry and western blot analysis.

Results: : Deficiencies in ephrin-A5-null lenses appear as early as P6, with 26% of the mutants developing large intercellular vacuoles in the fiber cell layer around the bow region. By P21, over 70% of ephrin-A5 mutants have developed large vacuoles between the fiber cells. The incidence rate increases to 86% by P180. Gross morphology of lenses during prenatal development appears normal. In contrast, no lens deformities are observed in the wild-type controls. Expression levels of several lens markers, including the crystallins and MIP-26, are also normal in ephrin-A5-null lenses.

Conclusions: : These findings indicate that ephrin-A5 activity is indispensible in postnatal lens development. While the absence of ephrin-A5 does not impair initial lens formation, the regulation of fiber cell-cell interaction requires ephrin-A5 activity for maintaining proper lens structure.

Keywords: cataract • development 
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