April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Shear and Modulus Measurements of Individual Corneal Epithelial Cells
Author Affiliations & Notes
  • S. S. Perry
    Materials Science and Engineering, R & D,
    University of Florida, Gainesville, Florida
  • J. Payne
    Materials Science and Engineering, R & D,
    University of Florida, Gainesville, Florida
  • T. Limpoco
    Materials Science and Engineering, R & D,
    University of Florida, Gainesville, Florida
  • H. A. Ketelson
    Materials Science and Engineering, R & D,
    Alcon Research Ltd, Fort Worth, Texas
  • D. L. Meadows
    Mechanical Engineering, Consumer Prod Rsch,
    Alcon Research Ltd, Fort Worth, Texas
  • W. G. Sawyer
    Mechanical Engineering, Consumer Prod Rsch,
    University of Florida, Gainesville, Florida
  • B. Keselowsky
    Biomedical Engineering,
    University of Florida, Gainesville, Florida
  • Footnotes
    Commercial Relationships  S.S. Perry, Alcon Laboratories, F; J. Payne, Alcon Laboratories, F; T. Limpoco, Alcon Laboratories, F; H.A. Ketelson, Alcon Laboratories, E; D.L. Meadows, Alcon Laboratories, E; W.G. Sawyer, Alcon Laboratories, F; B. Keselowsky, Alcon Laboratories, F.
  • Footnotes
    Support  Alcon Laboratories
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 4620. doi:
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    • Get Citation

      S. S. Perry, J. Payne, T. Limpoco, H. A. Ketelson, D. L. Meadows, W. G. Sawyer, B. Keselowsky; Shear and Modulus Measurements of Individual Corneal Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4620.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Living human corneal epithelial cells have been probed in vitro via atomic force microscopy, revealing the frictional characteristics and stiffness of the outer surfaces of mature corneal cells in media.

Methods: : Human corneal epithelial (HCE) cells from an immortalized cell line (HCE-T) were cultured under Dulbecco’s Modified Eagle Medium containing 10 ng/ml human epithelial growth factor (EGF). The cells were maintained under sterile cell media during experimentation.

Results: : Shear stresses of 0.40 kPa measured at the surface of individual corneal epithelial cells are among the lowest reported values for aqueous lubrication. The mechanical properties of individual epithelial cells have been further probed through nanometer scale indentation measurements. An elastic foundation model, based on experimentally verifiable parameters including cell diameter and thickness, is used to fit the indentation data, producing an effective elastic modulus of 16.5 kPa and highlighting the highly compliant nature of the cell surface. The elastic foundation model is found to more accurately fit the experimental data, to avoid unverifiable assumptions, and to produce a modulus significantly higher than that of the widely used Hertz-Sneddon model.

Conclusions: : The results provide a baseline understanding of the mechanical properties of individual corneal cells and again highlight the opportunities for probing the details of mechanobiology related to the function of the eye surface and for conducting interfacial/solution studies at the cellular level.

Keywords: cornea: epithelium • cell membrane/membrane specializations • cornea: basic science 
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