April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Measurement of Spatial Distribution of Corneal Elasticity With Acoustic Radiation Force Elasticity Microscope
Author Affiliations & Notes
  • E. R. Mikula
    Biomedical Engineering, University of California, Irvine, Mission Viejo, California
  • H. Sun
    Ophthalmology,
    University of California, Irvine, Irvine, California
  • K. Hollman
    Biomedical Engineering, University of Michigan, Ann Arbor, Michigan
  • J. V. Jester
    Ophthalmology,
    University of California, Irvine, Irvine, California
    Gavin Herbert Eye Institute, University of California, Irvine, Orange, California
  • T. Juhasz
    Ophthalmology,
    Biomedical Engineering,
    University of California, Irvine, Irvine, California
  • Footnotes
    Commercial Relationships  E.R. Mikula, None; H. Sun, None; K. Hollman, None; J.V. Jester, None; T. Juhasz, None.
  • Footnotes
    Support  NIH Grant EY018665
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 4630. doi:
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    • Get Citation

      E. R. Mikula, H. Sun, K. Hollman, J. V. Jester, T. Juhasz; Measurement of Spatial Distribution of Corneal Elasticity With Acoustic Radiation Force Elasticity Microscope. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4630.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

The non-uniform structure of the cornea suggests an inhomogeneous spatial distribution of corneal elasticity. Using the newly developed non-invasive acoustic radiation force elastic microscopy (ARFEM) we show that the anterior cornea is more rigid than the underlying posterior stromal bed in cadaver porcine eyes.

 
Methods:
 

Corneas from fresh porcine eyes (Sierra Medical, Whittier, CA) were excised from the globe leaving a 2 mm scleral rim intact. The corneal samples were suspended in collagen gelatin (10% w/w) within a water tank filled with deionized, degassed water. The water tank was attached to a 3-D mechanical stage allowing for precise control of cavitation bubble placement within the cornea. Femtosecond laser pulses induced optical breakdown and produced cavitation in the anterior and posterior cornea. A confocal ultrasonic transducer applied 6.5 ms acoustic radiation force-chirp bursts to the bubble at 1.5 MHz while monitoring bubble position using pulse-echoes at 20 MHz. A cross-correlation method was used to calculate bubble displacements. Maximum bubble displacements are inversely proportional to the Young’s modulus.

 
Results:
 

Bubble displacement was 18-25% greater in the posterior cornea relative to the anterior cornea. This indicates a larger Young’s modulus in the anterior cornea in the direction orthogonal to the corneal surface.

 
Conclusions:
 

Our non-invasive ARFEM results indicate in cadaver porcine eyes that the anterior cornea is stiffer than the posterior cornea.  

 
Keywords: cornea: stroma and keratocytes • cornea: basic science • refractive surgery 
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