Abstract
Purpose: :
To investigate the effect of anti-high mobility group box 1 (HMGB1) monoclonal antibody (mAb) against ischemia reperfusion injury in the rat retina.
Methods: :
Retinal ischemia was induced by increasing intraocular pressure to 130 mmHg and maintaining that level for 45 minutes. An intraperitoneal injection of HMGB1 was administered 30 min before ischemia. At 7 days after the ischemia, retinal damage was evaluated. Immunohistochemistry and image analysis were used to measure changes in the levels of reactive oxygen species (ROS) and the localization of anti-HMGB1 mAb. Dark-adapted full-field electroretinography (ERG) was also performed.
Results: :
Pretreatment with anti-HMGB1 mAb significantly enhanced the ischemic injury of the inner retina. Anti-HMGB1 mAb expression increased at 6 to 12 hours after ischemia in the inner retina. After the ischemia, production of ROS was detected in retinal cells. However, pretreatment with anti-HMGB1 mAb increased the production of ROS. On the seventh postoperative day, the amplitudes of the ERG b-waves were significantly higher in the vehicle group than in the groups pretreated with anti-HMGB1 mAb.
Conclusions: :
Anti-HMGB1 mAb plays a large deleterious role in ischemia-reperfusion injury in an in vivo model of retinal injury. Anti-HMGB1 mAb function should be further explored to develop neuroprotective therapeutic strategies for acute retinal ischemic disorders.
Keywords: ischemia • oxidation/oxidative or free radical damage • electroretinography: non-clinical