April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Identification of Radical Scavenging Compound From the Leaves of Phyllostachys nigra by Online HPLC-ABTS+ and Its Neuroprotective Effects Against Oxidative Stress in Retinal Ganglion Cells
Author Affiliations & Notes
  • S. Jung
    Natural Products Research Center, Korea Institute of Science & Technology, Gangneung, Republic of Korea
  • H. Lee
    Natural Products Research Center, Korea Institute of Science & Technology, Gangneung, Republic of Korea
  • K.-A. Kim
    Natural Products Research Center, Korea Institute of Science & Technology, Gangneung, Republic of Korea
  • E. Lee
    Natural Products Research Center, Korea Institute of Science & Technology, Gangneung, Republic of Korea
  • C. Kim
    Natural Products Research Center, Korea Institute of Science & Technology, Gangneung, Republic of Korea
  • B. Um
    Natural Products Research Center, Korea Institute of Science & Technology, Gangneung, Republic of Korea
  • Footnotes
    Commercial Relationships  S. Jung, None; H. Lee, None; K.-A. Kim, None; E. Lee, None; C. Kim, None; B. Um, None.
  • Footnotes
    Support  This work was supported by grant No.RTI05-01-02 from the Regional Technology Innovation Program of the Ministry of Knowledge Economy (MKE).
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 4742. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      S. Jung, H. Lee, K.-A. Kim, E. Lee, C. Kim, B. Um; Identification of Radical Scavenging Compound From the Leaves of Phyllostachys nigra by Online HPLC-ABTS+ and Its Neuroprotective Effects Against Oxidative Stress in Retinal Ganglion Cells. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4742.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : This study was to determine whether the compound isolated from Phyllostachys nigra, black bamboo could attenuate oxidative stress induced transformed retinal ganglion cells (RGC-5 cells) death.

Methods: : RGC-5 cells in culture were given two different insults such as l-buthionine-(S,R)-sulfoximine (0.5 mM) plus glutamate (10 mM) for 24 hours or hydrogen peroxide (300 µM for 24 hours), after which cell survival were tested. For a rapid assessment of pure antioxidant compound, online-HPLC-ABTS+ method were used. We isolated compounds from P. nigra using preparative RP-HPLC and Sephadex LH-20 column chromatography and elucidated chemical structures using 1H and 13C NMR. ROS quantification were performed by using the fluorescence probe, DCFH -DA, and we tested glutathione level based on the reaction of glutathione with 5,5’-dithio-bis(2-nitrobenzoic acid). Apoptotic cell death were measured by propidium iodide/Hoechst 33342 double staining and western blot analysis

Results: : Four systematic fractions from 95% ethanol extract were tested for protective effect in oxidative stress induced RGC-5 cell death. Among them, ethyl acetate fraction showed a significantly higher inhibition which was concentration dependent manner. The detected antioxidant (compounds 6) by online-HPLC-ABTS+ method and other seven compounds were isolated. Their chemical structures were elucidated as isoorientin (1), orientin (2), vitexin (3), p-coumaric acid (4), cis-coumaric acid (5), luteolin 6-C-(6''-O-trans-caffeoylglucoside) (6), vittariflavone (7) and tricin (8).The luteolin 6-C-(6''-O-trans-caffeoylglucoside) (compound 6) significantly attenuated the negative insult of BSO plus glutamate and hydrogen peroxide to RGC-5 cells. Treatment of the RGC-5 cells with compound 6 reduced the reactive oxygen species and recovered reduced glutathione level.Moreover, compound 6 attenuated apoptosis caused by two different models of oxidative stress.

Conclusions: : These results indicate that compound 6 isolated from P. nigra could be offered as leading compounds for retinal disease via antioxidative and antiapoptotic effects.

Keywords: neuroprotection 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×