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X. Mo, J. Dong; Screen Out of Chinese Traditional Medicines on the Effect of Promoting Axon Elongation and Regeneration to the Rat Retinal Tissue in Three-Dimensional Culture System. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4743.
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To screen out and compare of herb monomers (puerarin,tetramethylpyrazine,bilobalide-B and saffron-I )on the regeneration of retinal ganglion cells (RGCs)axons in vitro.
The retinal tissues of SD rats aged 1-3d were cut into pieces of 0.5mm×0.5mm ,and cultured in three-dimensional culture system . Investigated the effects to different concentrations of puerarin,tetramethylpyrazine,bilobalide-B and saffron-I (0.125g/l,0.25 g/l,0.5 g/l,1.0 g/l) on the growing of RGCs axons ,using fluorescence inverted microscopy and immunohistochemical staining. The number and length of outgrowing RGCs axons were calculated in days of 3,6,9.
The number and length of regrowing axons to all of the puerarin tetramethylpyrazine,bilobalide-B and saffron-I treated were larger than that in the control(13.3±1.5,6d),among which the effect to bilobalide-B(60±3.6,6d,0.25g/l )was strongest (p<0.05). Thy-1.1 and GAP-43 double positive fibers showed to be regenerated axons.
Puerarin,tetramethylpyrazine,bilobalide-B and saffron-I could improve the regeneration and elongation of cultured RGCs axons, and bilobalide-B is the most active.Retinal tissue three-dimensional culture system proved to be a good tool of evaluating retinal neuroregeneration effects for herb monomers.
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