Abstract
Purpose: :
To screen out and compare of herb monomers (puerarin,tetramethylpyrazine,bilobalide-B and saffron-I )on the regeneration of retinal ganglion cells (RGCs)axons in vitro.
Methods: :
The retinal tissues of SD rats aged 1-3d were cut into pieces of 0.5mm×0.5mm ,and cultured in three-dimensional culture system . Investigated the effects to different concentrations of puerarin,tetramethylpyrazine,bilobalide-B and saffron-I (0.125g/l,0.25 g/l,0.5 g/l,1.0 g/l) on the growing of RGCs axons ,using fluorescence inverted microscopy and immunohistochemical staining. The number and length of outgrowing RGCs axons were calculated in days of 3,6,9.
Results: :
The number and length of regrowing axons to all of the puerarin tetramethylpyrazine,bilobalide-B and saffron-I treated were larger than that in the control(13.3±1.5,6d),among which the effect to bilobalide-B(60±3.6,6d,0.25g/l )was strongest (p<0.05). Thy-1.1 and GAP-43 double positive fibers showed to be regenerated axons.
Conclusions: :
Puerarin,tetramethylpyrazine,bilobalide-B and saffron-I could improve the regeneration and elongation of cultured RGCs axons, and bilobalide-B is the most active.Retinal tissue three-dimensional culture system proved to be a good tool of evaluating retinal neuroregeneration effects for herb monomers.
Keywords: neuroprotection • drug toxicity/drug effects • nerve fiber layer