April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Evaluation of Collagen Derived Antiangiogenic Factors and Matrix Metalloproteinases in Lens Epithelial Cells of Pediatric Eyes with Persistent Hyperplastic Primary Vitreous (PHPV)
Author Affiliations & Notes
  • D. U. Gajjar
    R & D, Iladevi Cataract & IOL Res Ctr, Ahmedabad, India
  • B. Alapure
    R & D, Iladevi Cataract & IOL Res Ctr, Ahmedabad, India
  • A. Pal
    R & D, Iladevi Cataract & IOL Res Ctr, Ahmedabad, India
  • T. Parmar
    R & D, Iladevi Cataract & IOL Res Ctr, Ahmedabad, India
  • A. Arora
    R & D, Iladevi Cataract & IOL Res Ctr, Ahmedabad, India
  • S. Shah
    R & D, Iladevi Cataract & IOL Res Ctr, Ahmedabad, India
  • K. Johar
    R & D, Iladevi Cataract & IOL Res Ctr, Ahmedabad, India
  • A. Vasavada
    R & D, Iladevi Cataract & IOL Res Ctr, Ahmedabad, India
  • Footnotes
    Commercial Relationships  D.U. Gajjar, None; B. Alapure, None; A. Pal, None; T. Parmar, None; A. Arora, None; S. Shah, None; K. Johar, None; A. Vasavada, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 4753. doi:
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      D. U. Gajjar, B. Alapure, A. Pal, T. Parmar, A. Arora, S. Shah, K. Johar, A. Vasavada; Evaluation of Collagen Derived Antiangiogenic Factors and Matrix Metalloproteinases in Lens Epithelial Cells of Pediatric Eyes with Persistent Hyperplastic Primary Vitreous (PHPV). Invest. Ophthalmol. Vis. Sci. 2010;51(13):4753.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To measure mRNA levels of collagen derived antiangiogenic factors (arresten, canstatin, tumstatin, endostatin) and matrix metalloproteinases (MMP-2 and MMP-9) in lens epithelial cells (LEC’s) of pediatric patients with PHPV as cases and with age matched congenital cataract as controls

Methods: : Anterior capsule harboring LEC’s from pediatric cataract patients with (n=6) and without PHPV (n=6) were collected from operation theatre. Samples were immediately proceeded with RNA extraction and cDNA construction. Quantitative real time RT-PCR was performed to determine the mRNA levels of arresten, canstatin, tumstatin, endostatin, MMP-2, MMP-9. GAPDH and beta Actin. The mRNA levels were expressed as a ratio, using the delta-delta method for comparing the relative expression results between controls and PHPV cases. Non-parametric Mann-Whitney U test was applied for statistical evaluation and P values < 0.05 were considered statistically significant.

Results: : The relative mRNA levels of MMP-2, MMP-9, arresten, canstatin, tumstatin and endostatin in control samples were 1.11E-03±0.0009, 3.72E-04±0.0001, 1.48E-02±0.006, 1.49E-01±0.02, 5.51E-02±0.024 and 3.00E-04±0.0002 respectively. The relative mRNA levels of MMP-2, MMP-9, arresten, canstatin, tumstatin and endostatin in PHPV samples were 4.76E-03±0.002, 3.72E-04±0.0001, 6.20E-03±0.003, 3.25E-01±0.01, 1.70E-01±0.007 and 3.20E-03±0.003 respectively. The mRNA level of MMP-2, tumstatin and cantatin was 4, 2.5 and 2.3 times higher respectively in PHPV cases than control subjects. In contrast no change was observed in MMP-9 mRNA levels (p=0.82) and 1.6 times decreased levels of arresten mRNA(p=0.01) was observed.

Conclusions: : A significant difference in the mRNA levels of antiangiogenic factors (arresten, canstatin, tumstatin) and MMP-2 was found in the LEC’s with PHPV. These factors appear to play a pivotal role in maintaining the lens avascularity, which gets altered during the pathogenesis of PHPV.

Keywords: cataract • glycoconjugates/glycoproteins • transcription 
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