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M. Schlich, K. Lorenz, N. Boehm, C. Kramann, N. Pfeiffer, F. H. Grus; Analysis of IgM Autoantibody Patterns as Marker for Acute Phase Immunoreactions in Pseudoexfoliation Glaucoma. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4846.
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In previous studies significant alterations in IgG autoantibody patterns of pseudoexfoliation glaucoma patients (PEXG) could be demonstrated. However, reactivities of IgM autoantibodies, which may indicate acute immunological processes in the context of disease progression, have not been studied yet. Therefore, we analyzed IgM autoantibody patterns of PEXG patients compared to control subjects (CO). Additionally we included pseudoexfoliation syndrome patients (PEXS), showing no signs of glaucoma, in order to detect potential markers in patients who might switch from PEXS to PEXG.
Sera of patients with PEXG (n=45), PEXS (n=19) and normal controls with cataract (n=39) were used for antibody analysis. Analyses were performed using protein-microarrays, which were prepared by spotting 56 different ocular antigens onto microarray slides. Arrays were incubated with sera (1:250), and were treated with a fluorescence labeled anti-IgM antibody for visualization of the antibody-antigen-reactions. Emitted signals were digitized and spot intensities were compared using diverse statistical techniques, such as ANOVA and multivariate discriminant analysis.
The comparison of IgM autoantibody reactivities from PEXG and CO subjects revealed several significantly increased autoantibody reactivities in the glaucoma group, e.g. anti-α-1-Antitrypsin, anti-Glyceraldehyd-3-Phosphatase or anti-Protein-S100 (P<0.001). Interestingly, patterns from PEXS patients compared to those from CO subjects showed the same differences as PEXG for most autoantibodies, e.g. increased reactivities for anti-α-1-Antitrypsin or anti-Protein S100 (P<0.01). Furthermore, we found autoantibodies which differentiate between PEXG and PEXS like anti-γ-Synuclein, anti-heat shock protein 27 and anti-γ-Crystalin (P≤0.05).
The analysis of IgM autoantibody patterns revealed multiple significant shifts in immunoreactivities of PEXG and PEXS patients. Autoantibody intensities with strongly increased values were found in these patients, indicating acute immunological processes.
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