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L. E. Caspers, D. Makhoul, L. Schandené, M. Ducarme, C. Bruyns, F. Mascart, F. Willermain; Analysis of the Role of Latent Tuberculosis Immunity in Cd4+ Regulatory T Lymphocytes Level in Patients With Sight Threatening Autoimmune Uveitis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4847.
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It has been suggested that the immune responses in subjects with latent tuberculosis (LTB) may contribute to the development of certain form of autoimmune uveitis. As CD4+ regulatory T lymphocytes have been reported to be involved both in the pathogenesis of tuberculosis and autoimmune uveitis, we have investigated the proportions of these cells among circulating lymphocytes from patients presenting non-infectious uveitis with or without LTB
In this prospective study, patients with sight threatening uveitis underwent a standard diagnosis procedure, including tuberculin skin test (TST), QuantiFERON-TB Gold IT (QTF) and chest Xray. 17 patients were included after excluding those patients with an infectious uveitis or those who had received prior immunomodulatory treatment. Healthy subjects served as control. T regulatory cells were characterised and quantified by flow cytometry using the most commonly used markers, CD3,CD4,CD25, CD127, and FOXP3+.
The percentage of CD3+CD4+CD25highFOXP3+ lymphocytes was significantly higher in the uveitis group compared to the controls (1,85 +/- 1,17 versus 0,76 +/- 0,54 p=0.01). The same tendency was noted when adding a low expression of CD127 to the characterization of CD4+Treg cells even if in this case, the differences between the two groups were not significant. No differences were observed between TST + and TST - nor between QTF + or negative patients. However, only the TST - and/or QFT - subjects had a higher percentage of CD4+Treg cells compared to the controls whereas this was not the case for the uveitis patients with LTB.
Patients with an autoimmune uveitis without concomitant latent tuberculosis have a paradoxical higher percentage of circulating CD4+ regulatory T cells lymphocytes compared to controls, whereas this is not the case for the patients suffering from uveitis and presenting LTB.
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