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M. Y. Avila, J. L. Navia; Genipin Induces Crosslinking in Cornea. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4980.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate genipin a non uv system for crosslinking in porcine corneas
Corneal porcine eyes were crosslinked with genipin at 0.25% with a designed vehicle (Non UV) in a group with epithelium (n = 10) and in a group without epithelium (n=10), UV-Riboflavin (n=10), and as a positive control, total eyes were treated with glutaraldehide 0,1% and were compared with non treated eyes (10 eyes per group) Intraocular pressure was continuous monitored by a pressure sensor canulated to the anterior chamber and the volume was changed once a IOP of 10 mmHg was achieved, in 200ul steps. Changes in volume were correlated as a function of IOP and Ocular rigidity was calculated as a the exponential of polynomial quadratic fit.
Significant higher IOP elevation was observed in the globes were the cornea was stiffened with any method (genipin ,UV-Riboflabin and glutaraldehide) with (200ul) of volume increase, IOP changed as follow: genipin 17.9 mmHg, genipin without epithelium 19.0mmHg, UVCRX 18.8 mmHg, glutaraldehide 23.9mmHg, vs ctrol 14.7 mmHg, and 400ul genipin 29.9 mmHg, genipin without epithelium 31.5mmHg, UVCRX 26.0mmHg, glutaraldehide 37.3 mmHg vs ctrol 18.7 mmHg.Ocular ridigity was increased, 0.00501 (genipin 0.25% with epithelium), 0.00537 (genipin 0.25% without epithelium), 0.00423(UVCRX), and 0.0696 in glutaraldehide treated eyes vs 0.00218(ctrol), (p=0.001)
Genipin induced a significant stiffness of the cornea, The treatment may be induced with intact epithelium avoiding the usage of UV radiation. This study showed that stiffened corneas by any method induced substancially higher IOP elevations when ocular volume is increased. Those changes suggest that a acute increase of the volume of the intraocular fluid may increase IOP in crosslinked corneal eyes despite the system that could be used. Implications of this finding may sugest that corneal croslinked patients should be followed more nearly in terms of IOP changes
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