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M. Prabakar, N. Ziebarth, J. Dias, L. Lau, C. Rowaan, S. Ulhorn, F. Manns, S. Yoo, J.-M. Parel; Swelling of Corneas Treated With UVA and Riboflavin Assessed by Optical Coherence Tomography. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4999.
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© ARVO (1962-2015); The Authors (2016-present)
To quantify the degree of collagen crosslinking in treated and untreated human cadaver corneas using swelling experiments.
Three pairs of human cadaver eyes (age: 54-86 years, <8 days postmortem) were used for this study: the right eye was treated and the left eye served as the control. The corneal thickness was restored to physiological levels by placing the whole globe, cornea side down, in 25% Dextran for 24 hours. The epithelium was removed for all eyes using alcohol and a cotton swap. One drop of 0.1% riboflavin solution (10mg riboflavin-5-phosphate in 10mL Dextran 20% solution) was placed on the cornea of the right eye every 5 minutes for 30 minutes. The cornea was then irradiated for 30 minutes using UVA light (=378nm) with an irradiance of 3mW/cm2. During the irradiation, one drop of the riboflavin solution was placed on the cornea every 5 minutes. The cornea was then removed from both the treated and untreated eyes. The cornea was placed in a Petri dish with balanced salt solution. It was imaged every 5 minutes with a custom-built laboratory optical coherence tomography system. The central corneal thickness was calculated from the a-scans obtained.
The central corneal thickness of all treated corneas at time 0 was 12% to 18% lower than the contralateral control. An exponential asymptotic function was fit to the thickness versus time graphs. The asymptotic thickness of the treated cornea was lower than the control in two out of the three pairs, indicating increased resistance to water uptake.
The results suggest that crosslinking treatment with UVA light results in structural changes in the cornea that make it less susceptible to water uptake.Support: IMSD (JD); Florida Lions Eye Bank; NIH center grant P30-EY014801; Research to Prevent Blindness; Henri and Flore Lesieur Foundation (JMP).
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