April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Stabilizing in-vitro Corneal Shape Using Collagen Crosslinkers
Author Affiliations & Notes
  • M. Mattson
    Ophthalmology, University of California, San Francisco, Pasadena, California
    Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California
  • J. Huynh
    Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California
  • J. A. Kornfield
    Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California
  • D. M. Schwartz
    Ophthalmology-Sch of Med, Univ California - San Franscisco, San Francisco, California
  • Footnotes
    Commercial Relationships  M. Mattson, Visdex Corporation, I; Visdex Corporation, C; 20080114283, P; J. Huynh, None; J.A. Kornfield, Visdex Corporation, F; Visdex Corporation, I; 20080114283, P; D.M. Schwartz, 20080114283, P; Visdex Corporation, I.
  • Footnotes
    Support  NIH Grant EY017484, That Man May See
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 5002. doi:
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    • Get Citation

      M. Mattson, J. Huynh, J. A. Kornfield, D. M. Schwartz; Stabilizing in-vitro Corneal Shape Using Collagen Crosslinkers. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5002.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To evaluate the ability of collagen crosslinkers to stabilize the corneal shape, using an intact globe expansion method (GEM).

Methods: : Enucleated eyes from 2-3 week old New Zealand White rabbits underwent mechanical debridement of the corneal epithelium. The corneas were then treated with either 1) R/UVA - riboflavin/dextran drops and UVA (370 nm) irradiation, 2) EY - eosin y gel formulation and visible light irradiation (525 nm), or 3) Control - dulbecco’s phosphate buffered saline (DPBS) and no light treatment. Following treatment, eyes were immersed in a DPBS bath at 37 C, and mounted for determination of ocular expansion at an elevated pressure of 35 mm Hg; expansion of cornea and sclera was measured using digital photography.

Results: : Control eyes typically rupture through failure at the limbus after 6-9 hours of elevated pressure. Both EY and R/UVA eyes demonstrated significantly longer expansion times before rupture (> 15 hours, or no rupture), with failure typically occurring in the untreated sclera. The corneal perimeter (CP - a measure of the profile of the cornea) provides a quantitative descriptor of the corneal expansion. R/UVA and EY corneas resist expansion (CP increases ~0%) while control eyes expand (CP increases ~8%) in the 6 hours before control eye rupture. Within the first hour of elevated pressure expansion, there is significant difference between both R/UVA and Control, and EY and Control; however, there is no significant difference between R/UVA and EY during the experiment.

Conclusions: : Treatment of the cornea with either EY or R/UVA stabilizes the cornea, increasing resistance to expansion and rupture. Stabilization with EY and R/UVA is indistinguishable with the current testing method, indicating the possibility for equivalent efficacy as treatment modalities.

Keywords: cornea: basic science • sclera • keratoconus 

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