April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
A Rat Model to Study Choroidal Blood Flow
Author Affiliations & Notes
  • C. Strohmaier
    Dept. of Ophthalmology & Optometry, Paracelsus University, Salzburg, Austria
  • C. Runge
    Dept. of Ophthalmology & Optometry, Paracelsus University, Salzburg, Austria
  • F. Schroedl
    Dept. of Ophthalmology & Optometry, Paracelsus University, Salzburg, Austria
  • H. Brandtner
    Dept. of Ophthalmology & Optometry, Paracelsus University, Salzburg, Austria
  • B. Bogner
    Dept. of Ophthalmology & Optometry, Paracelsus University, Salzburg, Austria
  • G. Grabner
    Dept. of Ophthalmology & Optometry, Paracelsus University, Salzburg, Austria
  • H. A. Reitsamer
    Dept. of Ophthalmology & Optometry, Paracelsus University, Salzburg, Austria
  • Footnotes
    Commercial Relationships  C. Strohmaier, None; C. Runge, None; F. Schroedl, None; H. Brandtner, None; B. Bogner, None; G. Grabner, None; H.A. Reitsamer, None.
  • Footnotes
    Support  Fuchs Foundation, Adele Rabensteiner Foundation, Lotte Schwarz Chair and the PMU FFF
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 5010. doi:
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      C. Strohmaier, C. Runge, F. Schroedl, H. Brandtner, B. Bogner, G. Grabner, H. A. Reitsamer; A Rat Model to Study Choroidal Blood Flow. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5010.

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Abstract
 
Purpose:
 

To develop a rat model for continuous measurements of choroidal blood flow, intraocular pressure and arterial pressure with the possibility to manipulate perfusion pressure mechanically in order to investigate the regulatory mechanisms of choroidal blood flow.

 
Methods:
 

Male Brown-Norway rats were anesthetised with Pentobarbital Sodium (80 mg/kg) i.p., a tracheotomy was performed. The animal was respired with room air, the preset weight adjusted ventilator settings were adapted to keep the expired CO2 in at 40 mmHg. A catheter for blood pressure measurement was inserted into the right femoral artery and advanced to the aorta, another catheter was inserted into the right femoral vein for drug administration.A thoracotomy was performed through the 8th intercostal space and a hydraulic occluder was placed around the inferior caval vein to impede venous return to the heart and thereby lower blood pressure. Choroidal blood flow was measured with a custom-made, non-invasive laser Doppler flowmeter.

 
Results:
 

Blood pressure was lowered typically from 100 mmHg to 20 mmHg. Between 100 and 50 mmHg, blood flow remained virtually constant, between 50 mmHg and 20 mmHg, a linear relationship between blood pressure and blood flow was observed.

 
Conclusions:
 

The presented model is appropriate for the investigation of blood flow in the choroid of rats demonstrating the regulatory potential of the choroidal vasculature as shown in other species before.  

 
Keywords: blood supply • choroid 
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