Purchase this article with an account.
C. Strohmaier, C. Runge, F. Schroedl, H. Brandtner, B. Bogner, G. Grabner, H. A. Reitsamer; A Rat Model to Study Choroidal Blood Flow. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5010.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To develop a rat model for continuous measurements of choroidal blood flow, intraocular pressure and arterial pressure with the possibility to manipulate perfusion pressure mechanically in order to investigate the regulatory mechanisms of choroidal blood flow.
Male Brown-Norway rats were anesthetised with Pentobarbital Sodium (80 mg/kg) i.p., a tracheotomy was performed. The animal was respired with room air, the preset weight adjusted ventilator settings were adapted to keep the expired CO2 in at 40 mmHg. A catheter for blood pressure measurement was inserted into the right femoral artery and advanced to the aorta, another catheter was inserted into the right femoral vein for drug administration.A thoracotomy was performed through the 8th intercostal space and a hydraulic occluder was placed around the inferior caval vein to impede venous return to the heart and thereby lower blood pressure. Choroidal blood flow was measured with a custom-made, non-invasive laser Doppler flowmeter.
Blood pressure was lowered typically from 100 mmHg to 20 mmHg. Between 100 and 50 mmHg, blood flow remained virtually constant, between 50 mmHg and 20 mmHg, a linear relationship between blood pressure and blood flow was observed.
The presented model is appropriate for the investigation of blood flow in the choroid of rats demonstrating the regulatory potential of the choroidal vasculature as shown in other species before.
This PDF is available to Subscribers Only