April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Assessment of Retinal/Choroidal Blood Flow Using Laser Doppler Flowmetry (LDF) in Rats
Author Affiliations & Notes
  • S. Hetu
    University of Montreal, Montreal, Quebec, Canada
  • G. Cordahi
    University of Montreal, Montreal, Quebec, Canada
  • R. Couture
    University of Montreal, Montreal, Quebec, Canada
  • E. Vaucher
    University of Montreal, Montreal, Quebec, Canada
  • Footnotes
    Commercial Relationships  S. Hetu, None; G. Cordahi, None; R. Couture, None; E. Vaucher, None.
  • Footnotes
    Support  FRSQ Vision Research Network
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 5031. doi:
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    • Get Citation

      S. Hetu, G. Cordahi, R. Couture, E. Vaucher; Assessment of Retinal/Choroidal Blood Flow Using Laser Doppler Flowmetry (LDF) in Rats. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5031.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Laser Doppler Flowmetry (LDF) is a non-invasive technique commonly used to assess retinal and choroidal blood flow in humans. This study aimed at testing the usefulness of this technique for the assessment of retinal blood flow in rats under hypercapnia and after pharmacological interventions. The relative contribution of the choroid circulation in the LDF signal was determined after retinal arteries occlusion by photocoagulation.

Methods: : Male Wistar rats (200-250g) were anesthetised with urethane (1.2 mg/kg, i.p.), placed in a stereotaxic apparatus and their pupils dilated (atropine 1%). In these conditions, no ocular movements were observed. The LDF probe (Moors Instruments, 1 emitting fibre, 8 collecting fibres) was placed in contact with the cornea, parallel with the optic axis. Blood pressure and heart rate were monitored continuously and hematologic parameters were measured. Hypercapnia was induced by inhalation of CO2 (8 % in medical air) while adenosine (2µM), sodium nitroprusside (10 µM) and endothelin-1 (1µg/mL) were injected intravitreally (5 µL). The cornea was maintained humid with topical application of saline throughout the experiment.

Results: : Blood flow was significantly increased by hypercapnia (19%), adenosine (14%) and sodium nitroprusside (16%), yet decreased by endothelin-1 (11%). Photocoagulation of retinal arteries decreased by 33% from baseline blood flow values (P<0.001).

Conclusions: : Results show that LDF represents a suitable non-invasive technique to monitor online quantitative changes of retinal blood flow in response to pharmacological stimuli in vivo. This technique could be used to assess vasoreactivity of retinal vessels in ocular diseases.

Keywords: blood supply • retina 

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