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D. Xu, F. Dong, L. Zhong, L. Lee; Pilot Study of the Expression of Atrial Natriuretic Peptide in the Vitreous Humor and Epiretinal Membrane of the Patients With Proliferative Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5102.
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© ARVO (1962-2015); The Authors (2016-present)
To determine the level of atrial natriuretic peptide (ANP) in the vitreous humor and epiretinal membrane(ERM) of the patients with proliferative diabetic retinopathy (PDR) and evaluate the possibility that ANP could be involved in the process of PDR.
Thirty cases of vitreous humor samples from PDR patients (test group)and non-PDR(control group) patients were collected respectively. ANP was determined by radioimmunoassay(RIA) and the protein content was determined by the method of Bradford. Fifteen cases of ERM samples from PDR (test group)and idiopathic macular epiretinal membrane(control group) patients, respectively, were collected. The expression of ANP gene was determined by semiquantitative reserve transcription- polymerase chain reaction(RT-PCR). The resulting ANP amount was divided by the GAPDH amount to obtain a normalized value. ANP concentrations, protein concentrations, ANP/protein ratios and normalized value were analyzed between two groups by Mann-Whitney U test.
Mean ANP concentrations in test and control group were (68.23±16.65)pg/ml(range:40.11~99.56pg/ml,median:66.30pg/ml)and (38.45±7.62)pg/ml(range:30.10~57.32pg/ml, median:36.86pg/ml), respectively(P<0.01). Mean protein concentrations were (810.53±188.60)ug/ml(range,401.33~1361.70ug/ml, median: 786.58ug/ml)and(596.17±161.80)ug/ml(range:375.69~1136.37ug/ml,median: 570.04ug/ml),respectively(P<0.01). Mean ANP/Protein ratio were(0.09±0.02)(rang,0.05~0.16,median,0.09)and(0.07±0.02)(range,0.04~0.14,median,0.06), respectively(P<0.01). Mean peak values of ANP and GAPDH in test group were 188.5±53.6(range: 109.3~255.0,median:254.0)and 237.9±31.1(range: 162.2~255.0, median:254). Mean peak values of ANP and GAPDH in control group were 100.4±12.0(range: 79.2~122.0,median: 98.3) and 231.6±36.3(range: 113.8~255.0, median: 246.8). The mean ratio of ANP and GAPDH in test and control group was 0.8±0.3(range: 0.51~1.4,median: 0.8) and 0.4±0.1(range: 0.31~0.82,median: 0.4), respectively(P<0.01).
The level of ANP in PDR membranes and vitreous humor were significantly higher than that of non-PDR patients through semiquantitative RT-PCR and RIA. Our findings suggested a role of ANP in PDR.
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