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J. D. Barbosa, Sr., S. Morales, L. A. Arana, A. Gonzalez, M. S. Humayun, G. J. Chader; The Effect of Controlled Electrolysis in the Vitreous for Pre-Retinal Oxygenation. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5114.
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To measure intravitreal oxygen produced through controlled short term electrolysis.
Baseline ERG, OCT, FA and pre-retinal oxygen measurements were performed on the right eyes of normal pigmented rabbits. Twelve animals were divided randomly into 3 groups; control group without pathology (n=4 2/2), Central retinal vein occlusion (CRVO) treatment group (n=4 2/2), Pan Retinal Photocoagulation (PRP) treatment group (n=4 2/2). The CRVO was created by Laser with intravenous Rose Bengal Model. Each group was sub divided in 2; and only one received active oxygen-generating electrode implantation (Electrolysis) for 30 minutes at 200 µA. For the 3 groups, PO2 was measured 30 min after Electrolysis, then 60 min, 90 min, 48 hrs, 1 week and 2 weeks. The animals then underwent ERG, OCT, FA and euthanazation. The tissues were studied histologically in 5 µm thick sections stained with hematoxylin and eosin. This animal study was approved by the USC IACUC.
Preretinal oxygen measurements, Middle and retrolenticular Vitreous Oxygen were similar after electrolysis (table 1)
Controlled electrolysis of vitreous results in increased preretinal oxygenation without vitrectomy. The level of preretinal oxygen measured at the above time intervals is similar to that seen with PRP. If the procedure is proven safe and effective in the long term, our study may pave the way for fabrication of an implantable intraocular prosthesis for continuous vitreal oxygenation.
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