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Y. Zhou, X. Fan, X. Song, X. Xu, R. Jia; Alteration of miRNA Profiles by Ionizing Radiation in Uveal Melanoma Cells. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5138.
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© ARVO (1962-2015); The Authors (2016-present)
Radiation is the preferred treatment for most cases of Uveal Melanoma (UM).It disrupts cellular homeostasis through activates various survival and death signaling pathways in UM cells. Although microRNAs (miRNAs) have recently been recognized as important regulators of gene expression and play an important role in response to cellular stress. It is not well examed if miRNAs play any roles in UM cells response to radiation.Therefore,we investigated whether any changes in the expression profiles of miRNAs in UM cell line OM431 and use this approach to identify radiation-responsive miRNAs.
UM cells were exposed to radiation with10Gy. Total RNA was extracted and miRNA expression was determined by microarray. The expression levels of specific miRNAs were confirmed by quantitative real-time PCR (qRT-PCR) and statistically analyzed. The target mRNAs of radiation-responsive miRNAs were predicted with a target prediction tool.
Microarray analysis identified several miRNAs that exhibited more than 2-fold changes in their expression levels following exposure to radiation, qRT-PCR analysis of a subset of the miRNAs showed patterns of regulation as the microarray data. Target prediction for radiation-responsive miRNAs suggests that they target genes related to apoptosis, regulation of cell cycle.
In conclusion, these data suggest that miRNAs influenced significantly by radiation in the OM431 UM cell line model, and they maymay have important roles in the regulation of radiation responses.
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