April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Protein Macroarray Profiling of Serum Autoantibodies in Pseudoexfoliation Glaucoma
E. W. J. Dervan; H. Chen; S. Ho; N. Brummel; M. Haralambova; E. Gould; D. Wallace; J. H. M. Prehn; C. J. O'Brien; D. Murphy
Author Affiliations & Notes
  • E. W. J. Dervan
    Institute of Ophthalmology, Mater Misericordiae University Hospital, Dublin, Ireland
  • H. Chen
    Centre for Human Proteomics, Royal College of Surgeons in Ireland, Dublin, Ireland
  • S. Ho
    Institute of Ophthalmology, Mater Misericordiae University Hospital, Dublin, Ireland
  • N. Brummel
    Centre for Human Proteomics, Royal College of Surgeons in Ireland, Dublin, Ireland
  • M. Haralambova
    Centre for Human Proteomics, Royal College of Surgeons in Ireland, Dublin, Ireland
  • E. Gould
    Centre for Human Proteomics, Royal College of Surgeons in Ireland, Dublin, Ireland
  • D. Wallace
    Institute of Ophthalmology, Mater Misericordiae University Hospital, Dublin, Ireland
  • J. H. M. Prehn
    Centre for Human Proteomics, Royal College of Surgeons in Ireland, Dublin, Ireland
  • C. J. O'Brien
    Institute of Ophthalmology, Mater Misericordiae University Hospital, Dublin, Ireland
    Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland
  • D. Murphy
    Centre for Human Proteomics, Royal College of Surgeons in Ireland, Dublin, Ireland
  • Footnotes
    Commercial Relationships  E.W.J. Dervan, None; H. Chen, None; S. Ho, None; N. Brummel, None; M. Haralambova, None; E. Gould, None; D. Wallace, None; J.H.M. Prehn, None; C.J. O'Brien, None; D. Murphy, None.
  • Footnotes
    Support  The Glaucoma Foundation, New York
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 5220. doi:
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      E. W. J. Dervan, H. Chen, S. Ho, N. Brummel, M. Haralambova, E. Gould, D. Wallace, J. H. M. Prehn, C. J. O'Brien, D. Murphy; Protein Macroarray Profiling of Serum Autoantibodies in Pseudoexfoliation Glaucoma. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5220.

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Abstract

Purpose: : Complex repertoires of IgG autoantibodies have been detected against ocular antigens in patients with glaucoma. Our aim was to identify and characterize the IgG autoantibody repertoires in sera of patients with pseudoexfoliation glaucoma (PXFG) using protein macroarrays.

Methods: : Serum samples of 21 patients with PXFG and 19 age and sex matched controls were profiled on high-density colony protein macroarrays expressing His-tagged recombinant human proteins derived from a human fetal brain cDNA library. Statistically prevalent expression clones in the PXFG group were sequenced. mRNA expression of identified antigens was examined in the rat ganglion cell line (RGC-5) and human brain and optic nerve cDNA. The IgG immunoreactivity of the sera of 20 control and 26 PXFG patients to purified C6orf129 was analysed using a reverse enzyme-linked immunoassay. The gene expression profile of C6orf129 in 40 different tissues was analysed using tissue qPCR array plates.

Results: : We detected an increased prevalence among the PXFG patients of serum antibodies to seven proteins - C6orf129; stathmin-like 4; transmembrane protein 9 domain family, member B; fibroblast growth factor receptor 3; cleft lip and palate transmembrane protein 1; EH- domain containing protein 1 and eukaryotic translation elongation factor 2. All antigens are expressed in RGC-5 cells and cDNA from human brain and optic nerve with the exception of stathmin-like 4 in the RGC-5 cells. Patients with PXFG have increased anti-C6orf129 IgG immunoreactivity compared to controls (P<0.05). C6orf129 is ubiquitously expressed in all tissue particularly in those with a secretory role.

Conclusions: : Screening high-density protein arrays identifies unique antibody profiles that may potentially act as biomarkers for patients with PXFG. Further characterisation of this autoantibody repertoire may also provide new insights into the pathophysiology of PXFG.

Keywords: immunomodulation/immunoregulation • autoimmune disease • gene/expression 
© 2010, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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