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N. R. Harris, R. M. McElhatten, M. B. Willis; Extraintestinal Manifestations of Colonic Inflammation: Effect of Dextran Sodium Sulfate on the Electroretinogram. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5589.
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© ARVO (1962-2015); The Authors (2016-present)
Inflammatory bowel disease (IBD) induces manifestations beyond the intestine in some patients, including ocular inflammation and dysfunctional dark-adaptation and electroretinogram (ERG) signals. To our knowledge, this phenomenon has not been investigated in mouse models of IBD, such as the commonly used model of ingestion of dextran sodium sulfate (DSS).
C57BL/6 mice were given drinking water for 6 days with (N=6) or without (N=5) 5% 40 kD DSS to induce colonic inflammation. After 6 days, an ERG procedure using 7 scotopic flash intensities ranging from about -6 to -3 log cd s/m2 was performed on the dark-adapted anesthetized mice, with quantification of the a- and b-waves.
The amplitudes of the b-waves tended to decrease in the DSS mice by 13-44% compared to controls, with a 44% decrease at a flash intensity of -5.1 log cd s/m2 and a 33% decrease at a flash intensity of -4.2 log cd s/m2 both reaching statistical significance (p<0.05). No statistical differences were observed in a-wave amplitudes or implicit times of either a- or b-waves.
DSS-induced colonic inflammation in mice might provide useful data related to the retinal manifestations of IBD.Supported by NIH P01DK043785 (Project 2; NRH).
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