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M. Yu, M. E. Rayborn, J. G. Hollyfield, N. S. Peachey; Retinas in CCL2 Deficient Mice Are Vulnerable to Damage by Chronic Light Exposure. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5593.
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Chemoattractant chemokine ligand 2 (CCL2) can recruit choroidal macrophages to clean up retinal debris, and thereby reduce the inflammation response related to complement activation. This study examined whether the retinas in CCL2 deficient mice are more vulnerable to chronic light exposure.
CCL2-/- mice (n=12) and C57BL/6 (WT) mice (n=12) were assigned to one of two groups. The cyclic light (CL) groups were maintained under standard animal housing conditions (50 cd/m2 during the light cycle). The light exposure (LE) groups underwent an additional 2 hours of light exposure (9000 cd/m2) 10 days per month. ERG and histological analyses were performed at 6 and 12 months of age.
At 6 months of age, there was no difference in ERG responses between CCL2-/- and WT mice reared under the CL or LE condition. At 12 months of age, however, ERG amplitudes of CCL2-/- mice were reduced in comparison to those of WT mice in CL groups. In addition, responses of CCL2-/- LE mice were further reduced in comparison to CCL2-/- CL mice. Anatomical analysis revealed a number of defects in the CCL2-/- LE and CL retina, including loss of photoreceptor and RPE cells, which were more prevalent in the LE group, consistent with the ERG data.
CCL2 function is important for the long-term maintenance of the outer retina. The absence of CCL2 makes the mouse retina more susceptible to damage from intense light exposure.
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