April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Retinas in CCL2 Deficient Mice Are Vulnerable to Damage by Chronic Light Exposure
Author Affiliations & Notes
  • M. Yu
    Cole Eye Institute, Department of Ophthalmology, Cleveland Clinic Lerner College of Medicine, Cleveland, Ohio
  • M. E. Rayborn
    Cole Eye Institute, Department of Ophthalmology, Cleveland Clinic Lerner College of Medicine, Cleveland, Ohio
  • J. G. Hollyfield
    Cole Eye Institute, Department of Ophthalmology, Cleveland Clinic Lerner College of Medicine, Cleveland, Ohio
  • N. S. Peachey
    Cole Eye Institute, Department of Ophthalmology, Cleveland Clinic Lerner College of Medicine, Cleveland, Ohio
    Research Service, Cleveland Veterans Affairs Medical Center, Cleveland, Ohio
  • Footnotes
    Commercial Relationships  M. Yu, None; M.E. Rayborn, None; J.G. Hollyfield, None; N.S. Peachey, None.
  • Footnotes
    Support  NEI/NIH, the VA, AHAF, Research Center Grant from FFB, and an Unrestricted Grant from Research to Prevent Blindness.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 5593. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M. Yu, M. E. Rayborn, J. G. Hollyfield, N. S. Peachey; Retinas in CCL2 Deficient Mice Are Vulnerable to Damage by Chronic Light Exposure. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5593.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Chemoattractant chemokine ligand 2 (CCL2) can recruit choroidal macrophages to clean up retinal debris, and thereby reduce the inflammation response related to complement activation. This study examined whether the retinas in CCL2 deficient mice are more vulnerable to chronic light exposure.

Methods: : CCL2-/- mice (n=12) and C57BL/6 (WT) mice (n=12) were assigned to one of two groups. The cyclic light (CL) groups were maintained under standard animal housing conditions (50 cd/m2 during the light cycle). The light exposure (LE) groups underwent an additional 2 hours of light exposure (9000 cd/m2) 10 days per month. ERG and histological analyses were performed at 6 and 12 months of age.

Results: : At 6 months of age, there was no difference in ERG responses between CCL2-/- and WT mice reared under the CL or LE condition. At 12 months of age, however, ERG amplitudes of CCL2-/- mice were reduced in comparison to those of WT mice in CL groups. In addition, responses of CCL2-/- LE mice were further reduced in comparison to CCL2-/- CL mice. Anatomical analysis revealed a number of defects in the CCL2-/- LE and CL retina, including loss of photoreceptor and RPE cells, which were more prevalent in the LE group, consistent with the ERG data.

Conclusions: : CCL2 function is important for the long-term maintenance of the outer retina. The absence of CCL2 makes the mouse retina more susceptible to damage from intense light exposure.

Keywords: electroretinography: non-clinical • retina • transgenics/knock-outs 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×