April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Effect of High Glucose on Expression of a Novel Mitochondrial Transport Protein ABC-me in Rat Retinal Endothelial Cells
Author Affiliations & Notes
  • E. Beglova
    Medicine,
    Ophthalmology,
    Boston University School of Medicine, Boston, Massachusetts
  • C. Moore
    Ophthalmology,
    Boston University School of Medicine, Boston, Massachusetts
    University of Massachusetts School of Medicine, Worcester, Massachusetts
  • S. Roy
    Medicine,
    Ophthalmology,
    Boston University School of Medicine, Boston, Massachusetts
  • M. Liesa
    Medicine,
    Boston University School of Medicine, Boston, Massachusetts
  • O. Shirihai
    Medicine,
    Boston University School of Medicine, Boston, Massachusetts
  • Footnotes
    Commercial Relationships  E. Beglova, None; C. Moore, None; S. Roy, None; M. Liesa, None; O. Shirihai, None.
  • Footnotes
    Support  NEI, NIH Grant 1R01EY018218; Lions Eye Research Organization
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 5597. doi:
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    • Get Citation

      E. Beglova, C. Moore, S. Roy, M. Liesa, O. Shirihai; Effect of High Glucose on Expression of a Novel Mitochondrial Transport Protein ABC-me in Rat Retinal Endothelial Cells. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5597.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Introduction: : Objective: A novel mitochondrial ATP-binding cassette transporter (ABC-me) protein has been shown to protect against oxidative stress. Although ABC-me expression has been indentified in various tissues it is currently unknown whether ABC-me is expressed in the retina. The aim of the study is to determine whether ABC-me is expressed in retinal endothelial cells (RRECs), and whether high glucose alters ABC-me expression in these cells.

Methods: : To determine the effect of high glucose on ABC-me expression, rat retinal endothelial cells (RRECs) were grown in normal (5mM) glucose or high (30mM) glucose medium for 7 days. At the end of this period, cells were harvested, protein was isolated, and Western blot analysis performed using rabbit ABC-me primary antibody. After transfer of proteins, the membrane was stained with Ponceau-S and the densitometric values used for correcting protein amounts loaded in the gel lanes. To determine the presence of ABC-me in the retina, protein from Sprague Dawley rat retinas were subjected to Western blot analysis using the ABC-me antibody.

Results: : Western blot analysis showed that the RRECs express ABC-me protein. Similarly, Western blot analysis also showed that cells grown in high glucose medium exhibit significant inhibition of ABC-me protein levels by approximately 25% compared to those of cells grown in normal medium. In addition, the presence of ABC-me expression in the rat retina was confirmed by Western blot analysis.

Conclusions: : This study provides the first evidence of ABC-me protein expression in the retina, and in the retinal endothelial cells in culture, and that high glucose downregulates ABC-me expression. Since ABC-me is protective against oxidative stress, high glucose induced inhibition of ABC-me expression may contribute to mitochondrial dysfunction in diabetic retinopathy.

Keywords: diabetic retinopathy • mitochondria 
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