Purchase this article with an account.
N. Singh, L. Luo, J. M. Simonis, B. K. Ambati; Higher Levels of Soluble NRP-1 May be Associated With Lesser Corneal Neovascularization in MRL6/MPJ Mice Compared to Their Background Strain C57BL/6J Mice. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5701.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To determine whether soluble neuropilin-1 is elevated in MRL6/MpJ mice compared to their background strain C57BL/6J mice.
C57BL/6J and MRL6/MpJ mice corneas were injected with plasmid expressing SiRNA against soluble NRP. The corneas were imaged after every 3 days and harvested 10 days after injections. C57BL6 mice developed significant higher level of blood vessels compared to MRL/MpJ mice. Corneas were immunostained with the CD31 antibodies and confocal images were obtained using an Olympus confocal microscope. RNA was isolated from the MRL6/MpJ and C57BL/6 mice and Real time PCR was performed using primers designed against N- terminal region present in soluble NRP-1 and C-terminal end present only in membrane NRP-1.
Normal MRL6/MpJ mice had significant higher levels of soluble NRP 1 compared to background strain C57BL/6J mice (p=0.00018). Vascularization of cornea leads to significant decrease in soluble NRP-1 (p=0.0153) with a concurrent increase in membrane NRP-1 (p=0.0374) in C57BL/6J mice, while there was no significant change in levels of soluble NRP-1 is MRL6/MpJ mice.
Higher level of soluble NRP1 may be associated with a resistance to injury-induced corneal neovascularization in MRL6/MpJ mice.
This PDF is available to Subscribers Only