April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Higher Levels of Soluble NRP-1 May be Associated With Lesser Corneal Neovascularization in MRL6/MPJ Mice Compared to Their Background Strain C57BL/6J Mice
Author Affiliations & Notes
  • N. Singh
    Ophthalmology-University of Utah, John Moran Eye Center, Salt Lake City, Utah
  • L. Luo
    Ophthalmology-University of Utah, John Moran Eye Center, Salt Lake City, Utah
  • J. M. Simonis
    Ophthalmology-University of Utah, John Moran Eye Center, Salt Lake City, Utah
  • B. K. Ambati
    Ophthalmology-University of Utah, John Moran Eye Center, Salt Lake City, Utah
  • Footnotes
    Commercial Relationships  N. Singh, None; L. Luo, None; J.M. Simonis, None; B.K. Ambati, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 5701. doi:
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      N. Singh, L. Luo, J. M. Simonis, B. K. Ambati; Higher Levels of Soluble NRP-1 May be Associated With Lesser Corneal Neovascularization in MRL6/MPJ Mice Compared to Their Background Strain C57BL/6J Mice. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5701.

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Abstract

Purpose: : To determine whether soluble neuropilin-1 is elevated in MRL6/MpJ mice compared to their background strain C57BL/6J mice.

Methods: : C57BL/6J and MRL6/MpJ mice corneas were injected with plasmid expressing SiRNA against soluble NRP. The corneas were imaged after every 3 days and harvested 10 days after injections. C57BL6 mice developed significant higher level of blood vessels compared to MRL/MpJ mice. Corneas were immunostained with the CD31 antibodies and confocal images were obtained using an Olympus confocal microscope. RNA was isolated from the MRL6/MpJ and C57BL/6 mice and Real time PCR was performed using primers designed against N- terminal region present in soluble NRP-1 and C-terminal end present only in membrane NRP-1.

Results: : Normal MRL6/MpJ mice had significant higher levels of soluble NRP 1 compared to background strain C57BL/6J mice (p=0.00018). Vascularization of cornea leads to significant decrease in soluble NRP-1 (p=0.0153) with a concurrent increase in membrane NRP-1 (p=0.0374) in C57BL/6J mice, while there was no significant change in levels of soluble NRP-1 is MRL6/MpJ mice.

Conclusions: : Higher level of soluble NRP1 may be associated with a resistance to injury-induced corneal neovascularization in MRL6/MpJ mice.

Keywords: cornea: basic science • neovascularization • protein structure/function 
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