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K. Sugisaki, T. Usui, N. Honda, S. Yokoo, S. Amano; Involvement of Leukemia Inhibitory Factor (lif) in Corneal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5705.
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Purposes: Leukemia inhibitory factor (LIF), a member of the interleukin 6 (IL-6) family, is widely known as a critical molecule for the maintenance of ES cells in culture. Recently it was reported that LIF modulate the vascular development in retina, suggesting LIF may play important roles in ocular angiogenesis. In this study we examined the expression of LIF in corneal neovascularization (NV).
Corneal NV was induced in C57/B6 mice by placing an intrastromal nylon suture 1 mm away from the limbus. Eyeballs were enucleated 1,3 and 7 days after suturing. LIF and its receptor (LIFR) expression were evaluated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), and immunohistochemistry.
LIF and LIFR mRNA markedly increased in the neovascularized corneas compared with normal corneas. LIF protein slightly expressed in stromal cells and endothelium of normal cornea, and its expression was apparent in stromal cells of neovascularized corneas. Otherwise, LIFR broadly expressed in the endothelium and basal cell layer of epithelium, and its expression was also stimulated after surgery.
These findings indicate that LIF, abundantly expressed in neovascularized corneas, may play a significant role in inflammation-related corneal neovascularization.
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