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C. Zhang, S. L. Hose, J. S. Zigler, Jr., D. Sinha; Modulation of Aquaporin 4 (AQP4) in the Nuc1 Spontaneous Mutant Rat With Persistent Fetal Vasculature (PFV). Invest. Ophthalmol. Vis. Sci. 2010;51(13):5756.
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© ARVO (1962-2015); The Authors (2016-present)
To understand the etiology of PFV in the Nuc1 mutant rat.
The Nuc1 spontaneous mutant rat and age-matched Sprague-Dawley wildtype (wt) rats were euthanized at 10, 20 and 75 days after birth. For immunohistochemistry with single or double labeling, frozen sections were incubated with primary antibodies, and then secondary fluorescent antibodies conjugated with either Cy-2 or Cy-3. The primary antibodies used were a monoclonal to AQP4, polyclonal GFAP and polyclonal Kir 4.1. Real-time PCR analysis was used for quantitation of AQP4 expression.
Our data indicates that βA3/A1-crystallin modulates, directly or indirectly, the expression of AQP4 in retinal astrocytes. The data also suggest that the increased AQP4 expression could lead to the abnormal migration of Nuc1 astrocytes and perhaps cause them to ensheath the hyaloid artery. Interestingly, a role for AQP4 in the migration of astrocytes has been reported earlier.
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