April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Indocyanine Green (ICG) Induces Increased Müller Cell Expression of Adhesion Molecules and Membrane Metalloproteinases in vitro
Author Affiliations & Notes
  • S. K. McRee
    Schepens Eye Research Institute, Boston, Massachusetts
  • B. Tucker
    Schepens Eye Research Institute, Boston, Massachusetts
    Ophthalmology, Harvard Medical School, Boston, Massachusetts
  • M. J. Bradbury
    Retina Consultants of Worcester, Worcester, Massachusetts
  • M. Young
    Schepens Eye Research Institute, Boston, Massachusetts
    Ophthalmology, Harvard Medical School, Boston, Massachusetts
  • G. Velez
    Schepens Eye Research Institute, Boston, Massachusetts
    Ophthalmology, Harvard Medical School, Boston, Massachusetts
  • Footnotes
    Commercial Relationships  S.K. McRee, None; B. Tucker, None; M.J. Bradbury, None; M. Young, None; G. Velez, None.
  • Footnotes
    Support  NIH 5KO8EY17383, Minda de Gunzburg Research Center for Retinal Transplantation, the Discovery Eye and Lincy Foundations, and Research to Prevent Blindness
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 5762. doi:
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    • Get Citation

      S. K. McRee, B. Tucker, M. J. Bradbury, M. Young, G. Velez; Indocyanine Green (ICG) Induces Increased Müller Cell Expression of Adhesion Molecules and Membrane Metalloproteinases in vitro. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5762.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : ICG is a protein often used during vitreoretinal surgical procedures to help visualize the internal limiting membrane (ILM). It has been shown in previous studies to induce apoptosis in vitro in Müller cells. The bcl-2 and caspase cascades have been suggested as possible pathways. However, Müller cells have also been shown to be stimulated in the context of retinal injury. This has been observed in animal models of both laser injury and retinal detachment. We explore the effect of ICG on Müller cells in vitro, and whether it can have a protracted and lasting stimulatory effect on these cells.

Methods: : Human Müller cells (MIO-M1) were exposed to clinical doses of ICG (2.5mg/ml) for 10 min, then thoroughly washed with PBS. Cells were harvested for lysate preparation immediately, 24 hrs, 48 hrs, and 72 hrs after exposure. Lysates were then analyzed by Western blotting for expression of the Integrins β1, α3, and α5, as well as MMP1, MMP2 and TIMP-1.

Results: : An acute increase in Integrin β1, Integrin α3, Integrin α5, MMP1, MMP2 and TIMP-1 was noted immediately after exposure to ICG. 24 hrs after exposure there was a sustained increase in expression when compared to control with a decrease when compared to expression immediately after exposure to ICG. 48 and 72 hrs after exposure, there was a sustained increase in expression above control and 24 hr levels.

Conclusions: : Our results suggest that the injury exerted by ICG on Müller cells has a sustained proliferative effect. This alteration in Müller cell behavior could lead to an increased gliotic response which could be beneficial in certain clinical situations (macular hole closure) but potentially detrimental in others.

Keywords: Muller cells • drug toxicity/drug effects • retina 
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