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H. Sen, B. Liu, L. Wei, S. Chakrabatry, R. B. Nussenblatt; Double Negative T Cells in Uveitis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5779.
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Behcet’s disease (BD) and sarcoidosis are systemic disorders that can be associated with uveitis. We aimed to identify the frequency of a group of T-cells, double negative T cells (DNT) in patients with BD, sarcoidosis and uveitis. DNT cells have been suggested to have effector inflammmatory phenotype however their role in patients with autoimmune disorders is poorly understood.
Human peripheral blood mononuclear cells (PBMCs) were isolated from blood using a Ficoll gradient centrifugation protocol. DNT were stained as CD3+CD4-CD8- and analyzed by a FACSCaliber flow cytometry. A total of 76 patients were evaluated for their DNT frequency. Thirty nine patients had uveitis, 20 patients had sarcoidosis and 17 patients had Behcet’s disease with or without ocular inflammation. Enzyme-linked immunosorbent assays were used to measure the cytokine expression.
The results showed that DNT constituted a small subset of lymphocytes both in patients and normals. Compared with normal subjects and patients with uveitis not associated with any known systemic disorder, patients with BD and sarcoidosis had significantly increased levels of DNT within the total lymphocyte population (0.99+/-0.21% and 2.5+/-2.6% vs 7.25+/-4.52% and 6.89+/-8.25%). DNT cells were capable of producing IL1, IL2, IL6, IL23, IL12, IL17 and TNF alpha whereas IL4, IL10 and IFNgamma appeared relatively low.
These results suggest that circulating DNT may be playing a critical role in patients with uveitis, particularly those associated with Behcet’s disease and sarcoidosis.
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