April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Soluble CD44 Increases Outflow Resistance in Porcine Organ Cultures
Author Affiliations & Notes
  • M. C. Giovingo
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois
  • R. D. McCarty
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois
  • R. M. Beverley
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois
  • M. J. Nolan
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois
  • J. R. Samples
    Ophthalmology, Casey Eye Institute, Portland, Oregon
  • B. Y. J. T. Yue
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois
  • P. A. Knepper
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois
    Department of Ophthalmology, Northwestern University, Chicago, Illinois
  • Footnotes
    Commercial Relationships  M.C. Giovingo, None; R.D. McCarty, None; R.M. Beverley, None; M.J. Nolan, None; J.R. Samples, None; B.Y.J.T. Yue, None; P.A. Knepper, Alcon, F.
  • Footnotes
    Support  Alcon Research Ltd.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 5838. doi:
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    • Get Citation

      M. C. Giovingo, R. D. McCarty, R. M. Beverley, M. J. Nolan, J. R. Samples, B. Y. J. T. Yue, P. A. Knepper; Soluble CD44 Increases Outflow Resistance in Porcine Organ Cultures. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5838.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

CD44 plays major roles in multiple physiological processes.The soluble CD44 (sCD44) concentration is significantly increasedin the aqueous humor of primary open-angle glaucoma (POAG).Increased sCD44 in mouse eyes causes an increase in intraocularpressure (IOP). The purpose of this study was to determine ifsCD44 changes outflow resistance in porcine anterior segmentorgan culture and profiles of lipid raft proteins of the trabecularmeshwork (TM).

 
Methods:
 

sCD44 was purified from human serum using anion exchange, hyaluronicacid (HA) affinity chromatography and immunoprecipitation. Anteriorsegments of porcine eyes were placed in organ culture and perfusedwith Dulbecco’s modified eagle medium (DMEM). Flow rateswere measured in eyes treated with sCD44, HA or DMEM. Perturbationof lipid raft containing proteins was assessed by Optiprep densitygradient and Western blot analysis of dissected TM.

 
Results:
 

Flow rates are expressed as percent change (+/-) from the baseline.Rates significantly decreased from baseline in HA and sCD44infused eyes. Analysis of lipid raft containing proteinsof sCD44 infused porcine microdissected TMs and primary culturesof TM cells revealed a decrease in annexin 2 and an increasein caveolin-1 compared to that of controls. 

 

 
Conclusions:
 

Infusion of sCD44 significantly decreased outflow facility inporcine eyes. Notably, sCD44 caused a decrease in annexin 2and an increase in caveolin-1 which may influence outflow resistanceand the regulation of IOP.

 
Keywords: outflow: trabecular meshwork • anterior segment • aqueous 
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