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R. W. Kuijpers, J. Bastiaans, W. Dik, A. Roefs, G. Baarsma, M. Pescatori, P. Van hagen; Anterior Chamber Aqueous Humor Analysis in Tuberculous Uveitis With a 507 Protein Array. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5900.
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Uveitis is an inflammatory state in the eye caused by different mechanisms. The purpose of this study is to identify and compare the presence of inflammatory proteins in the aqueous humour of patients with uveitis due to tuberculosis.
Anterior chamber taps were performed as a diagnostic procedure in uveitis patients. Work-up consisted of a PPD skin test and a Quantiferon test. 4 patients were diagnosed with active TBC. As a control, aqueous humour of patients undergoing a cataract extraction was used. Samples were applied to Ray-Bio human cytokine array-L series: 507 proteins (Ray Biotech).
More than 10-fold change was found for Fibroblast growthfactor receptor 4 (FGF-R4), Oncostatin M (OSM), Estrogen down regulated gene-1 (EDG-1), CC Chemokine receptor 3 (CCR3), Single immunoglobulin domain containing Il-1R related protein (SIGIRR), Neuroregulin-2 (NRG2), Tomoregulin-1 and Il-19 (all p< 0.0002); Four or more-fold down regulation was found for CC Chemokine receptor 4 (CCR4), urokinase plasminogen activator receptor (uPAR/CD87) and Tumour necrosis factor receptor 1 (TNF-R1).
Increased levels of proteins were found that are involved in oncogenic pathways, besides inflammatory pathways. Novel pathways and proteins that have not been associated with uveitis sofar, were identified. Moreover, with this technique profiles can be generated to distinguish different kinds of inflammation.Authors: Kuijpers RWAM, Dik W, Bastiaans J, Pescatori M, Baarsma GS, Roefs A and Van Hagen PM
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