April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Effect of Anti-VEGF Therapy on Intraocular Biochemical Profile in Neovascular Conditions
Author Affiliations & Notes
  • R. K. Sharma
    Ophthalmology, University of Florida, Jacksonville, Florida
  • A. T. Rogojina
    Molecular Biology, 2St. Jude Children’s Research Hospital, Memphis, Tennessee
  • K. V. Chalam
    Ophthalmology, University of Florida, Jacksonville, Florida
  • Footnotes
    Commercial Relationships  R.K. Sharma, None; A.T. Rogojina, None; K.V. Chalam, None.
  • Footnotes
    Support  Deans Grant, Departmental
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 5905. doi:
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      R. K. Sharma, A. T. Rogojina, K. V. Chalam; Effect of Anti-VEGF Therapy on Intraocular Biochemical Profile in Neovascular Conditions. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5905.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Vascular endothelial growth factor (VEGF) plays a key role in neovascularization by stimulating proliferation and migration of vascular endothelial cells. Anti-VEGF therapy, bevacizumab, acts by binding to VEGF and preventing its effects. However, this linier interaction represents only a partial view of pathobiology of neovascular diseases and the anti VEGF treatment. To obtain an integrated view of the processes involved in ocular neovascularization we applied systems approach, and investigated whether intravitreal bevacizumab injection had a global effect in normalizing the ocular physiology perturbed by the disease.

Methods: : Vascular endothelial growth factor (VEGF) plays a key role in neovascularization by stimulating proliferation and migration of vascular endothelial cells. Anti-VEGF therapy, bevacizumab, acts by binding to VEGF and preventing its effects. However, this linier interaction represents only a partial view of pathobiology of neovascular diseases and the anti VEGF treatment. To obtain an integrated view of the processes involved in ocular neovascularization we applied systems approach, and investigated whether intravitreal bevacizumab injection had a global effect in normalizing the ocular physiology perturbed by the disease.

Results: : 43 analytes were detected above the sensitivity of the assay both in pre and post injection samples. Of these normal values of 41 analytes was known and these analytes were further analyzed. Detected analytes included relevant markers such as such as VEGF, C reactive protein, glutathione and cytokines. We identified 22 markers that were perturbed more than 1.5 fold in diseased samples (pre injection) as compared to the normal levels. The levels of perturbed analytes were compared in post treatment samples. Results demonstrate an unequivocal trend towards normalization in post treatment samples.

Conclusions: : 43 analytes were detected above the sensitivity of the assay both in pre and post injection samples. Of these normal values of 41 analytes was known and these analytes were further analyzed. Detected analytes included relevant markers such as such as VEGF, C reactive protein, glutathione and cytokines. We identified 22 markers that were perturbed more than 1.5 fold in diseased samples (pre injection) as compared to the normal levels. The levels of perturbed analytes were compared in post treatment samples. Results demonstrate an unequivocal trend towards normalization in post treatment samples.

Keywords: aqueous • retinal neovascularization • cytokines/chemokines 
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