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A. Ortin-Martinez, M. Jiménez-López, F. Nadal-Nicolás, L. Alarcón-Martínez, L. Nieto-López, Y. Sauve, M. Vidal-Sanz, M. P. Villegas-Pérez, M. Agudo; Automatic Quantification of the Total Number of S and M/l Cones and Their Detailed Topography in Albino and Pigmented Adult Rats. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5946.
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To quantify the whole population of short wavelength cones (S cones) and long-medium wavelength cones (M/L cones) and to study their topographical distribution in the albino (Sprague Dawley, SD) and pigmented (Pieblad Virol Glax, PVG) rats.
Whole mounted retinas were double immunodetected with antibodies against UV-sensitive opsin and opsin red/green to detect the S and M/L cones, respectively. An automatic method was developed to quantify the whole population of each type cone. Detailed topographical distribution of S and M/L cones was observed with isodensity maps based upon the quantification data. Mirror distribution of retinal ganglion cells (RGC) and M/L cones was achieved by double immunodetection of Brn3a and Opsin red/green, respectively
The mean number±SEM of S cones in albino and pigmented strain is 41,028±5,074 and 27,316± 2,235, respectively. In SD retinas there are 231,736±14,517 M/L cones and in PVG ones 239,939±6,494. In the S cones distribution is observed an increasing density gradient along the infero-nasal quadrant, though their highest densities are found in the retinal rim. The topography of S and M/L cones seems to be complementary. The highest densities of M/L cones are observed in the superior naso-temporal axis, paralleling the distribution of Brn3a positive RGCs
These data ascertain, for the first time, the total number and the topographical distribution of S and M/L cones in two rat strains and demonstrate the correlation of M/L cones and RGC spatial distribution
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