April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Role of Fat Protocadherins in Early Eye Development & Optic Fissure Closure
Author Affiliations & Notes
  • S. Bharti
    NEI/NIH, Bethesda, Maryland
  • I. F. Onojafe
    NEI/NIH, Bethesda, Maryland
  • J. D. Brown
    NEI/NIH, Bethesda, Maryland
  • H. McNeill
    Molecular Genetics, Mount Sinai Hospital, University of Toronto, Ontario, Canada
  • B. P. Brooks
    NEI/NIH, Bethesda, Maryland
  • Footnotes
    Commercial Relationships  S. Bharti, None; I.F. Onojafe, None; J.D. Brown, None; H. McNeill, None; B.P. Brooks, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 5958. doi:
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    • Get Citation

      S. Bharti, I. F. Onojafe, J. D. Brown, H. McNeill, B. P. Brooks; Role of Fat Protocadherins in Early Eye Development & Optic Fissure Closure. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5958.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : We have previously noted that Fat4, a member of the protocadherin adhesion molecule family, is dynamically regulated at the closing edges of the optic fissure during eye development, suggesting a potential role in the pathogenesis of a potentially-blinding ocular malformation, uveal coloboma. Here we investigate the role of Fat cadherins in early ocular development (EOD) and optic fissure closure (E10.5-E12.5) in mouse.

Methods: : Fat protocadherin mRNA expression was evaluated in mouse at three developmental time points using RT-PCR and in situ hybridization. The ocular phentoype of Fat1-/-and Fat4-/-mice was examined histologically and molecularly.

Results: : Fat1

Conclusions: : Fat cadherins are dynamically regulated in time and space during EOD, with expression patterns consistent with having a role in optic fissure closure. Ongoing work includes molecular characterization of both mutant mouse lines and assessing the role of Fat1/Fat4 in the fusion of the neuroepithelium at the optic fissure.

Keywords: cell adhesions/cell junctions • development • genetics 

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