Abstract
Purpose: :
Ocular albinism type 1 (OA1) is characterized by abnormalities in retinal pigment epithelium (RPE) melanosomes and misrouting of optic axons. This disease is caused by mutations in the OA1 gene, which encodes a melanosomal G-protein coupled-receptor (GPCR). We have shown before that Gαi3 signals in the same pathway as OA1. The purpose of this study is to demonstrate that Gαi3 interacts with Oa1.
Methods: :
Pull-down
Results: :
Pull-down
Conclusions: :
Our in vitro experiments corroborate our previous studies that showed that Oa1 and Gαi3 signal in the same pathway demonstrating that Gαi3 protein specifically binds to the GPCR Oa1.
Keywords: retinal pigment epithelium • receptors • fluorescent in situ hybridization