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X. Chen, J. Wang, J. Lu, H. Shen, D. Hu, D. Yan; MicroRNA-1 Inhibits Uveal Melanoma Cell Proliferation and Migration. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5990.
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MicroRNAs (miRNAs) are 20-24 nucleotide non-coding RNAs which are expressed endogenously and repress protein translation through binding to target mRNAs. Evidence indicates that miR-1 is essential for cardiac development and play important roles in rhabdomyosarcoma development. The function of miR-1 in uveal melanoma, however, remains unknown. In the present study, we investigated the function of MicroRNA-1 (miR-1) in uveal melanoma cells.
Realtime PCR was performed to detect the expression of miR-1 in uveal melanoma cells. miR-1 was transfected into uveal melanoma cells by liperfectamine 2000. Cell proliferation was measured by MTS assay. Cell cycle analysis was performed by flow cytometry. The migration of melanoma cells was examined by transwell migration assays. Western blot analysis was carried out to detect the expression of c-Met in uveal melanoma cells.
miR-1 expression was upregulated in uveal melanoma cells after treated with DNA demethylating agents. Ectopic expression of miR-1 induced G1-arrest in uveal melanoma cells. Transfection of miR-1 into uveal melanoma cells significantly inhibited cell proliferation and migration. miR-1 downregulated c-Met expression in uveal melanoma cells.
Our results demonstrated that miR-1 may act as a tumor suppressor in uveal melanoma cell proliferation and migration.
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