April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
MicroRNA-34a Inhibits Human Corneal Epithelial Cell Proliferation and Migration
Author Affiliations & Notes
  • D. Yan
    Sch of Ophthal & Optometry, Wenzhou Medical College, Wenzhou Zhejiang, China
  • C. Lu
    Sch of Ophthal & Optometry, Wenzhou Medical College, Wenzhou Zhejiang, China
  • J. Zhu
    Sch of Ophthal & Optometry, Wenzhou Medical College, Wenzhou Zhejiang, China
  • X. Chen
    Sch of Ophthal & Optometry, Wenzhou Medical College, Wenzhou Zhejiang, China
  • J. Wang
    Sch of Ophthal & Optometry, Wenzhou Medical College, Wenzhou Zhejiang, China
  • L. Tu
    Sch of Ophthal & Optometry, Wenzhou Medical College, Wenzhou Zhejiang, China
  • Footnotes
    Commercial Relationships  D. Yan, None; C. Lu, None; J. Zhu, None; X. Chen, None; J. Wang, None; L. Tu, None.
  • Footnotes
    Support  National Natural Science Foundation of China (30772385)
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 5991. doi:
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    • Get Citation

      D. Yan, C. Lu, J. Zhu, X. Chen, J. Wang, L. Tu; MicroRNA-34a Inhibits Human Corneal Epithelial Cell Proliferation and Migration. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5991.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : MicroRNAs (miRNAs) are endogenous short (~22) nucleotide RNAs which repress protein translation through binding to a target mRNA. Recent studies have shown that miR-34a can regulate cell proliferation and migration of tumor cells. The role of miR-34a in human corneal epithelial cells, however, remains unclear. In the present study, we investigated the function of microRNA-34a (miR-34a) in human corneal epithelial cells.

Methods: : In situ hybridization was performed to detect the expression of miR-34a in human corneal epithelium. MTS and wound-healing assay was carried out to evaluate the proliferation and migration of human corneal epithelial cells, respectively. The target of miR-34a was predicted by bioinformatics and confirmed by luciferase assay. Human corneal epithelial cells were transfected with miR-34a. The expression of c-Met protein was determined by Western blotting.

Results: : miR-34a was expressed in human corneal epithelium. Transfection of miR-34a into human corneal epithelial cells led to a significant decrease in cell proliferation and migration. c-Met was demonstrated to be a direct target of miR-34a in human corneal epithelial cells by bioinformatics and luciferase assay. miR-34a was found to downregulate the expression of c-Met protein by Western blot analysis.

Conclusions: : Our results demonstrated that miR-34a inhibited human corneal epithelial cell proliferation and migration by targeting c-Met. This indicates that miR-34a may play an important role in corneal wound healing process.

Keywords: cornea: epithelium • wound healing • gene/expression 
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