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D. Ding, M. Algecirs, T.-H. Chou, V. Porciatti, S. K. Bhattacharya; Retinal Deimination and Functional Status in a Mice Model of Multiple Sclerosis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5995.
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To demonstrate that a transgenic mice model (ND4) of multiple sclerosis undergoes modulation in protein deimination in the retina and functional status of the inner retina compared to littermate controls.
Transgenic mice model (ND4) of multiple sclerosis overexpressing proteolipid protein (PLP) and non-transgenic control mice (n=10 each) were used for these studies. Expanded disability status scale (EDSS) as well as magnetic resonance imaging (MRI) of the brain and upper spinal cord was used to score the disease. Western and immunohistochemical analysis was performed to determine the posttranslational deimination in the retina and flash and pattern electroretinogram (FERG and PERG) was used to determine retinal function.
The transgenic ND4 mice showed altered level of deimination in the retina together with significant impairment of inner retinal function as demonstrated by weakened signal in the PERG (but not FERG). Significant deimination was observed in the retinal ganglion cell prior to the onset of disability on EDSS and demonstrable changes in the cerebellum with MRI.
We have found hypo-deimination of retinal ganglion cell layer and significant decrease in inner retinal function in the ND4 multiple sclerosis mice.
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