April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Stromal Derived Factor-1 in Rhegmatogenous Retinal Detachment
Author Affiliations & Notes
  • H. Otsuka
    Kagoshima University, Kagoshima, Japan
  • N. Arimura
    Department of Ophthalmology, Kagoshima University, Kagoshima City, Japan
  • K. Yamakiri
    Kagoshima University, Kagoshima, Japan
  • T. Hashiguchi
    Laboratory Medicine,
    Kagoshima University, Kagoshima, Japan
  • I. Maruyama
    Laboratory Medicine,
    Kagoshima University, Kagoshima, Japan
  • T. Sakamoto
    Ophthalmology, Kagoshima Univ Sch of Medicine, Kagoshima-shi, Japan
  • Footnotes
    Commercial Relationships  H. Otsuka, None; N. Arimura, None; K. Yamakiri, None; T. Hashiguchi, None; I. Maruyama, None; T. Sakamoto, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 5999. doi:
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    • Get Citation

      H. Otsuka, N. Arimura, K. Yamakiri, T. Hashiguchi, I. Maruyama, T. Sakamoto; Stromal Derived Factor-1 in Rhegmatogenous Retinal Detachment. Invest. Ophthalmol. Vis. Sci. 2010;51(13):5999.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Stromal cell drived factor-1 (SDF-1) is essential for angiogenesis, embryogenesis, and tissue repair through circulating bone marrow-derived stem cells. Several studies have reported a neuroprotective role of SDF-1. However, that has been elusive in ocular diseases. In this study, we assessed the role of SDF-1 in rhegmatogenous retinal detachment (RRD) using human vitreous samples and rat experimental retinal detachment (RD) model.

Methods: : The study involved 78 eyes of 78 cases which received vitrectomy: 49 regmatogenous retinal detachment, 18 macular hole (MH), and 11 epiretinal membrane (ERM). Vitreous samples were collected before vitrectomy. The amount of SDF-1 levels was measured by enzyme-linked immunosorbant assay (ELISA). Other cytokines, such as interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12, or Tumor necrosis factor-α were quantified by cytometric bead array. In vivo experiment, immediately after induction of RD, we performed intravitreal injection of anti SDF-1 antibody alone or in combination with recombinant IL-6 (rIL-6), then we observed the retinal section at 7 days after RD.

Results: : RRD had a median SDF-1 concentration of 58.5 pg/ml, MH had that of 10.0 pg/ml and ERM had that of 21.2pg/ml. SDF-1 concentraion of RRD was significantly higher than that of ERM (P=0.012, mann-whitney test). SDF-1 concentration of RRD was significantly correlated with both duration of disease and extent of detached retina, respectively (P=0.025, P=0.040, spearman rank correlation test). In addition, the concentration of IL-6 and IL-8 of RRD were higher, which were significantly correlated with the concentration of SDF-1. The IL-6 concentration of RRD was significantly correlated with extent of detached retina, but no significant correlation was found with duration of disease (P=0.025, P=0.742, spearman rank correlation test). In the rat experimental RD, the blocking of vitreous SDF-1 resulted in significant loss of outer nuclear layer thickness at 7 days after RD (P<0.001, versus control, student's t test). That was significantly reduced by addition of rIL-6 (P=0.038, versus anti SDF-1 antibody, student's t test).

Conclusions: : SDF-1 might have an important role in maintenance of photoreceptor in RD, its mechanism might be related to IL-6.

Keywords: retinal detachment • vitreous • retina 

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