Abstract
Purpose: :
Stromal cell drived factor-1 (SDF-1) is essential for angiogenesis, embryogenesis, and tissue repair through circulating bone marrow-derived stem cells. Several studies have reported a neuroprotective role of SDF-1. However, that has been elusive in ocular diseases. In this study, we assessed the role of SDF-1 in rhegmatogenous retinal detachment (RRD) using human vitreous samples and rat experimental retinal detachment (RD) model.
Methods: :
The study involved 78 eyes of 78 cases which received vitrectomy: 49 regmatogenous retinal detachment, 18 macular hole (MH), and 11 epiretinal membrane (ERM). Vitreous samples were collected before vitrectomy. The amount of SDF-1 levels was measured by enzyme-linked immunosorbant assay (ELISA). Other cytokines, such as interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12, or Tumor necrosis factor-α were quantified by cytometric bead array. In vivo experiment, immediately after induction of RD, we performed intravitreal injection of anti SDF-1 antibody alone or in combination with recombinant IL-6 (rIL-6), then we observed the retinal section at 7 days after RD.
Results: :
RRD had a median SDF-1 concentration of 58.5 pg/ml, MH had that of 10.0 pg/ml and ERM had that of 21.2pg/ml. SDF-1 concentraion of RRD was significantly higher than that of ERM (P=0.012, mann-whitney test). SDF-1 concentration of RRD was significantly correlated with both duration of disease and extent of detached retina, respectively (P=0.025, P=0.040, spearman rank correlation test). In addition, the concentration of IL-6 and IL-8 of RRD were higher, which were significantly correlated with the concentration of SDF-1. The IL-6 concentration of RRD was significantly correlated with extent of detached retina, but no significant correlation was found with duration of disease (P=0.025, P=0.742, spearman rank correlation test). In the rat experimental RD, the blocking of vitreous SDF-1 resulted in significant loss of outer nuclear layer thickness at 7 days after RD (P<0.001, versus control, student's t test). That was significantly reduced by addition of rIL-6 (P=0.038, versus anti SDF-1 antibody, student's t test).
Conclusions: :
SDF-1 might have an important role in maintenance of photoreceptor in RD, its mechanism might be related to IL-6.
Keywords: retinal detachment • vitreous • retina