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A. K. Yu, K. I. Forward, S. A. Morales, L. K. Gordon, L. S. Morse, S. S. Park, D. G. Telander; Co-Expression of RPE65 and GFAP With EMP2 in Human Proliferative Vitreoretinopathy (PVR) Membranes. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6089.
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Previous studies have found that Epithelial Membrane Protein 2 (EMP2) is highly expressed in retinal pigment epithelial (RPE) cells and regulates cell contraction. In addition, our previous studies have also shown that EMP2 is expressed in human epiretinal membranes from proliferative vitreoretinopathy (PVR). RPE cells are known to play a significant role in these epiretinal membranes. The cells in the PVR membranes including the RPE are known to undergoing trans-differentiation into other cell phenotypes. The purpose of this study is to beter characterize the EMP2 positive cells in the PVR membranes including determining glial fibrillary acidic protein (GFAP) and RPE65 expression.
Proliferative vitreoretinopathy (PVR) membranes were collected from patients with consent. The PVR membranes were fixed and processed for paraffin embedding. The protein expression of EMP2, RPE65 and GFAP was evaluated by immunohistochemical analysis.
Initial qualitative analysis shows varied expression of RPE65 and GFAP amongst tissue samples. RPE65 is expressed to a lesser extent than EMP2. GFAP expression appears to correlate with PVR activity, but again to a lesser extent than EMP2. The percentage of EMP2 positive cells is consistently higher in all severe PVR membranes to than both RPE65 and GFAP expressing cells.
The decreased expression of RPE65 compared to EMP2 suggests that RPE65 may be down regulated before EMP2 during their trans-differentiation. This data further supports the role of EMP2 in the development of human PVR, and emphasizes the need for further study into this potential therapeutic target for PVR.
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