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A. W. Krysta, M. Zenkel, J. Djordjevic-Jocic, P. Lewczuk, A. G. Junemann, F. E. Kruse, J. Kornhuber, U. Schlotzer-Schrehardt; MMP/TIMP Profiles in the Aqueous Humor of Glaucoma Patients: Effect of Antiglaucoma Drugs. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6113.
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Matrix metalloproteases (MMPs) and their tissue inhibitors (TIMPs) are implicated in the pathophysiology of glaucoma. We investigated the concentrations of MMPs and TIMPs in the aqueous humor of patients with pseudoexfoliation glaucoma (PEXG) and primary open-angle glaucoma (POAG) without and with antiglaucoma medications as well as of control patients with cataract.
Fluorokine MultiAnalyte Profiling kits and Luminex technology were used to simultaneously measure MMP-1/-2/-3/-7/-9/-12/-13 and TIMP-1/-2/-3/-4 in aqueous humor samples obtained from patients with cataract, PEXG and POAG without or with antiglaucomatous drug therapy (latanoprost or timolol/dorzolamide) for at least 12 months (n≥10 for each group) during cataract or glaucoma surgery after informed consent. Data were statistically analyzed by the Mann-Whitney test.
MMP-1/-2/-3/-7/-9 and TIMP-1/-2/-3/-4 were identified in human aqueous humor samples from all groups of patients with highest levels for MMP-2 and TIMP-2, whereas MMP-12 and -13 were below detection limit. Compared with cataract patients, all four TIMPs were significantly increased in aqueous samples from both untreated and treated PEXG and POAG patients, whereas MMP-2 and -7 were increased in untreated PEXG patients only. Compared with untreated glaucoma patients, treatment with latanoprost resulted in a significant increase in aqueous MMP-1 levels of both PEXG and POAG patients as well as a reduction of MMP-7 levels in PEXG patients and a reduction of TIMP-1 and -4 in POAG patients only. Glaucoma medication had no significant effect on aqueous levels of MMP-2/-3/-9 and TIMP-2/-3.
Increased aqueous concentrations of TIMPs were confirmed to be associated with both PEXG and POAG. Latanoprost therapy significantly influenced aqueous concentrations of MMP-1 and TIMP-1/4 levels and may confer beneficial effects on the abnormal matrix accumulations in both types of glaucoma.
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