April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
T Cell Receptors Are Associated With Retinal Ganglion Cell Death
Author Affiliations & Notes
  • T. Kanamoto
    Ophthalmology & Visual Science, Hiroshima Univ Sch of Med, Hiroshima, Japan
  • Y. Kiuchi
    Ophthalmology & Visual Science, Hiroshima Univ Sch of Med, Hiroshima, Japan
  • Footnotes
    Commercial Relationships  T. Kanamoto, None; Y. Kiuchi, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 6122. doi:
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      T. Kanamoto, Y. Kiuchi; T Cell Receptors Are Associated With Retinal Ganglion Cell Death. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6122.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : A proteomic study from our laboratory showed that the expression of the protein of T cell receptor beta (TCRβ) was changed in the retina of DBA2/J mice, a mouse model of glaucoma (Proteomics, 9(21):4962-9. 2009). The purpose of this study was to determine whether various type of TCRs, including TCRα, -β, -γ, and -Δ, are expressed in the retina of mice, and whether they are associated with the death of retinal ganglion cell death (RGCs) induced by glutamate neurotoxicity and oxidative stress.

Methods: : The retinal sites of expressing TCRs were determined by immunohistochemistry with specific antibodies for TCRs in the retina of mice. Alternative changes of the protein expression of TCRs 7-months-of-age to 11-month-of age in the retina of DBA2/J mice were measured by western blot analysis. MTS assays were performed to investigate the effect of siRNA of TCRs on the death of rat retinal ganglion cells, RGC-5 cells, induced by the exposure to excess glutamate or hydrogen peroxide.

Results: : All of TCRs, TCRα, -β,-γ, and -Δ were expressed in the RGCs of mice. While the expression of TCRβ in the retina at 11-month-of age of DBA2/J mice was lower than at 7-month-of age, TCRα,TCRγ,and TCRΔ were higher. The death of RGC-5 cells induced by glutamate toxicity was promoted by siRNA of TCRβ, but that was reduced by siRNAs of TCRα or TCRγ. RGC-5 death by hydrogen preroxide was inhibited by siRNAs of TCRα, TCRβ, or TCRγ.

Conclusions: : These results suggest that TCRs are associated with the death of RGCs.

Keywords: immunomodulation/immunoregulation • retinal degenerations: cell biology • neuroprotection 

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