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T. Kanamoto, Y. Kiuchi; T Cell Receptors Are Associated With Retinal Ganglion Cell Death. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6122.
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© ARVO (1962-2015); The Authors (2016-present)
A proteomic study from our laboratory showed that the expression of the protein of T cell receptor beta (TCRβ) was changed in the retina of DBA2/J mice, a mouse model of glaucoma (Proteomics, 9(21):4962-9. 2009). The purpose of this study was to determine whether various type of TCRs, including TCRα, -β, -γ, and -Δ, are expressed in the retina of mice, and whether they are associated with the death of retinal ganglion cell death (RGCs) induced by glutamate neurotoxicity and oxidative stress.
The retinal sites of expressing TCRs were determined by immunohistochemistry with specific antibodies for TCRs in the retina of mice. Alternative changes of the protein expression of TCRs 7-months-of-age to 11-month-of age in the retina of DBA2/J mice were measured by western blot analysis. MTS assays were performed to investigate the effect of siRNA of TCRs on the death of rat retinal ganglion cells, RGC-5 cells, induced by the exposure to excess glutamate or hydrogen peroxide.
All of TCRs, TCRα, -β,-γ, and -Δ were expressed in the RGCs of mice. While the expression of TCRβ in the retina at 11-month-of age of DBA2/J mice was lower than at 7-month-of age, TCRα,TCRγ,and TCRΔ were higher. The death of RGC-5 cells induced by glutamate toxicity was promoted by siRNA of TCRβ, but that was reduced by siRNAs of TCRα or TCRγ. RGC-5 death by hydrogen preroxide was inhibited by siRNAs of TCRα, TCRβ, or TCRγ.
These results suggest that TCRs are associated with the death of RGCs.
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