April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Red Blood Cell Levels of Choline-Phospholipids as a Mirror of Retinal and Optic Nerve Compositions: Human Data
N. Acar; O. Berdeaux; L. Martine; G. Thuret; S. Cabaret; S. Gregoire; P. Gain; C. P. Garcher; A. M. Bron; L. Bretillon
Author Affiliations & Notes
  • N. Acar
    INRA, University of Burgundy, Eye & Nutrition Research Group, Dijon, France
  • O. Berdeaux
    INRA, University of Burgundy, Eye & Nutrition Research Group, Dijon, France
    INRA, University of Burgundy, Lipid-Aroma Platform, Dijon, France
  • L. Martine
    INRA, University of Burgundy, Eye & Nutrition Research Group, Dijon, France
  • G. Thuret
    University Hospital, Biology, Engineering and Imaging of Corneal Grafts, JE2521, University Jean Monnet, Saint Etienne, France
  • S. Cabaret
    INRA, University of Burgundy, Eye & Nutrition Research Group, Dijon, France
    INRA, University of Burgundy, Lipid-Aroma Platform, Dijon, France
  • S. Gregoire
    INRA, University of Burgundy, Eye & Nutrition Research Group, Dijon, France
  • P. Gain
    University Hospital, Biology, Engineering and Imaging of Corneal Grafts, JE2521, University Jean Monnet, Saint Etienne, France
  • C. P. Garcher
    INRA, University of Burgundy, Eye & Nutrition Research Group, Dijon, France
    Ophthalmology, University Hospital, Dijon, France
  • A. M. Bron
    INRA, University of Burgundy, Eye & Nutrition Research Group, Dijon, France
    Ophthalmology, University Hospital, Dijon, France
  • L. Bretillon
    INRA, University of Burgundy, Eye & Nutrition Research Group, Dijon, France
  • Footnotes
    Commercial Relationships  N. Acar, None; O. Berdeaux, None; L. Martine, None; G. Thuret, None; S. Cabaret, None; S. Gregoire, None; P. Gain, None; C.P. Garcher, None; A.M. Bron, None; L. Bretillon, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 6124. doi:
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      N. Acar, O. Berdeaux, L. Martine, G. Thuret, S. Cabaret, S. Gregoire, P. Gain, C. P. Garcher, A. M. Bron, L. Bretillon; Red Blood Cell Levels of Choline-Phospholipids as a Mirror of Retinal and Optic Nerve Compositions: Human Data. Invest. Ophthalmol. Vis. Sci. 2010;51(13):6124.

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Abstract

Purpose: : The lipid composition of red blood cell membranes is currently admitted to reflect that of nervous tissues, including the retina and the optic nerve. We have shown previously specific alterations of red blood cell levels of individual species of phosphatidyl-cholines (PC) and plasmenyl-cholines (PlsC) in primary open angle glaucoma (POAG) patients (Acar et al, Exp Eye Res 2009: 840-853). In order to assign these circulating modifications to ocular changes, the aim of this work was to identify in humans, plausible associations in the levels PC and PlsC between red blood cells, retina and optic nerve.

Methods: : Red blood cells, retinas and optic nerves were collected from nine donors (body donation to Science). Following lipid extraction, total phospholipids were isolated using silica cartridges. The individual species of PC and PlsC were quantified by HPLC-ESI-MS/MS on a triple quadrupole MS instrument. Correlations were estimated for each compound to determine plausible associations between circulating and tissue concentrations.

Results: : Several associations were found between red blood cell and retinal and/or optic nerve levels of individual species of PC and PlsC. For PlsC, the associations were mainly with the optic nerve whereas they were restricted to the retina for PC. PlsC18:1/22:4 in red blood cells was positively associated with PlsC18:1/16:0 in the optic nerve (r=0.75, p=0.04) and PlsC18:0/22:4 with PlsC18:1/22:4 and PlsC18:1/20:2 (r=0.82, p=0.02 and r=0.91, p=0.004, respectively). On the other hand, PC18:0/22:6 in red blood cells was positively associated with PC18:1/22:6 and PC22:6/22:6 in the retina (r=0.67, p=0.003 and r=0.74, p=0.01, respectively).

Conclusions: : The red blood cell concentrations of some individual species of PlsC and PC appear to reflect their levels in the optic nerve and in the retina, respectively. Since PlsC are known to be concentrated in myelin and PC in retinal cells, the alteration of their levels in red blood cells of POAG patients may suggest optic nerve demyelinisation and retinal cell loss processes.

Keywords: lipids • retina • optic nerve 
© 2010, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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